Table 2 Primers used in this study
From: SimReg1 is a master switch for biosynthesis and export of simocyclinone D8 and its precursors
Primer | Nucleotide sequence (5'-3') | Purpose | Gene name |
|---|---|---|---|
SSR1F | ATACCATGGCCCGTGAACGT | SimReg1 | simReg1 |
SSR1R | TTTGAATTCATTAATGGTGATGGT | purification | |
SR1D4F | TAGAATTCGTGAGCAGATCATGT | DNA-shift assay | P D4 |
SR1D4R | TAGAATTCCATTGTGAACCATC | ||
SD2R1F | TAGAATTCATCGCCACGACCATG | DNA-shift assay | P R1 |
SD2R1R | TAGAATTCCGCGGTTCGGCAGA | ||
simX5D3F | TAGAATTCTGTACAAGGCCTGGT | DNA-shift assay | P D3 |
simX5D3R | TAGAATTCGCGACAGGAGCCATA | ||
simEXX4F | TAGAATTCGACGCCTTCCAGTC | DNA-shift assay | P X4 |
simEXX4R | TAGAATTCTCAGAACATCGTCC | ||
SR2ExXF | AAATCTAGATCAAGCCAGTGCTG | DNA-shift assay | P R2Ex |
SR2ExXR | TTTGAATTCTTGACCACCACTTC | ||
PSR2F | TCGACGAGGTCCTCTTTG | DNA-shift assay | P SR2 |
PSR2R | TCGTATTCATACACCGTAC | ||
PEx1F | CCAATTGCGCTACGCTCCT | DNA-shift assay | P SEx1 |
PEx1R | CCATGTAGGCGGTGACGA | ||
simA7F | TAAAGCTTCAAAACGGGGTGAAC | DNA-shift assay | P A7 |
simA7R | ATAAGCTTGTCGATACCGATCTTC | ||
PEx2F | ACTTCCCAGAAGTA | DNA-shift assay | P Ex2 |
PEx2R | AGAGGGCAGTAGAC | ||
PR3F | TTTCTAGATGCACCCGATCCTC | DNA-shift assay | P SR3 |
PR3R | GAACAGGATTCGCATGAGTACT | ||
D4For | TATTGGTCGCGCAGTCGTCC | DNA-shift assay | part of the simD4 gene |
D4Rev | TTGTCCTGCATCATGACGAG | ||
simD4_for_script | AAATCTAGAGGCGACCGACCCCG GCCGAG | simD4 promoter cloning | P D4 |
simD4_rev_script | AAAGGTACCCGATCCGGCTGGCA TTACTG | ||
simReg1_for | AAAAAGCTTTACCTGTATCCAGGGC GGGCACTT | simReg1 cloning | simReg1 |
simReg1_ rev | AAAGGATCCGCACAAAGCGGCAGC AATCG |