Figure 8
From: SimReg1 is a master switch for biosynthesis and export of simocyclinone D8 and its precursors
Results of an EMSA performed to investigate the influence of crude extracts from S. antibioticus ü6040 × pSSimR1-1 strain on the interactions of SimReg1 to promoter regions of the sim cluster. In "a" promoter P R1 and in "b" P A7 were used. (a) lane 1: P R1 ; lane 2: P R1 + His-SimReg1; lane 3: P R1 + His-SimReg1 + crude extract isolated from S. antibioticus Tü6040 × pSSimR1-1 (5% of total reaction volume); lane 4: P R1 + His-SimReg1 + crude extract isolated from S. antibioticus Tü6040 × pSSimR1-1 (10% of total reaction volume); lane 5: P R1 + His-SimReg1 + methanol (5% of total reaction volume); lane 6: P R1 + His-SimReg1 + methanol (10% of total reaction volume); (b) lane 1: P A7 ; lane 2: P A7 + His-SimReg1; lane 3: P A7 + His-SimReg1 + crude extract isolated from S. antibioticus Tü6040 × pSSimR1-1 (5% of total reaction volume); lane 4: P A7 + His-SimReg1 + crude extract isolated from S. antibioticus Tü6040 × pSSimR1-1 (10% of total reaction volume)