Table 1 Purification scheme for HQDO from Sphingomonas sp. strain TTNP3
From: Purification and characterization of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3
Purification step | Activity (U) | Protein (mg) | Spec. act. (U mg-1) | Purification factor | Yield (%) |
|---|---|---|---|---|---|
Cell extract | 35.7 | 245 | 0.15 | 1 | 100 |
Ammonium sulfate fractionation | 35.9 | 108 | 0.33 | 2.3 | 101 |
Phenyl-Sepharose | 34.5 | 19.2 | 1.80 | 12.4 | 89 |
DEAE | 14.8 | 3.3 | 4.42 | 30.4 | 44 |
MonoQ | 9.5 | 1.6 | 6.06 | 41.6 | 30 |