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Table 1 Purification scheme for HQDO from Sphingomonas sp. strain TTNP3

From: Purification and characterization of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3

Purification step Activity (U) Protein (mg) Spec. act. (U mg-1) Purification factor Yield (%)
Cell extract 35.7 245 0.15 1 100
Ammonium sulfate fractionation 35.9 108 0.33 2.3 101
Phenyl-Sepharose 34.5 19.2 1.80 12.4 89
DEAE 14.8 3.3 4.42 30.4 44
MonoQ 9.5 1.6 6.06 41.6 30