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AMB Express

Table 4 Thermal properties of PHA containing 3H4MV synthesized by the mutant 1F2 using leucine as a 3H4MV precursor, P(3HB-co-3HV), and P(3HB-co-3HHx)

From: Enhanced Incorporation of 3-Hydroxy-4-Methylvalerate Unit into Biosynthetic Polyhydroxyalkanoate Using Leucine as a Precursor

  

PHA composition a

Thermal property

Molecular weight

Polymer

Leucine (g/L)

3HV (mol%)

3H4MV (mol%)

3HHx (mol%)

Total b (mol%)

T m (°C)

T g (°C)

Δ H m (J/g)

M n (×10 3 )

M w/M n

P(3HB-co-3HV-co-3H4MV)c

0

1.6

0.8

0

2.4

146, 159

3

42

250

1.9

P(3HB-co-3HV-co-3H4MV)c

5

2.3

1.0

0

3.3

142, 154

3

40

251

1.8

P(3HB-co-3HV-co-3H4MV)c

10

1.2

3.1

0

4.3

137, 151

3

42

98

1.6

P(3HB) d

-

0

0

0

0

172

4

77

224

2.1

P(3HB-co-3HV) e

-

8

0

0

8

170

-

70

-

-

P(3HB-co-3HHx) f

-

0

0

5

5

151

0

69

100

1.9

  1. M n, number-average molecular weight; M w, weight-average molecular weight; M w/M n; polydispersity index; T m, melting temperature; T g, glass-transition temperature; ΔH m, enthalpy of fusion.
  2. a PHA compositions of purified copolymer samples were determined by GC. Copolymer compositions other than 3HB are shown.
  3. b 3HV plus 3H4MV plus 3HHx fraction.
  4. c PHA synthesized by mutant 1F2 from fructose (20 g/L) and leucine (0, 5, 10 g/L).
  5. d P(3HB) homopolymer synthesized by R. eutropha H16.
  6. e (Scandola et al. 1992).
  7. f (Doi et al. 1995).
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