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AMB Express

Table 3 PHA biosynthesis by R. eutropha PHB-4 expressing phaC1 Ps or leucine analog resistant mutants with the supplementation of 10 g/L leucine

From: Enhanced Incorporation of 3-Hydroxy-4-Methylvalerate Unit into Biosynthetic Polyhydroxyalkanoate Using Leucine as a Precursor

 

Dry cell weight (g/L)

PHA content (wt%)

PHA composition (mol%) a

Strain

  

3HB

3HV

3H4MV

Parent b

7.2

29

98.8

0.3

0.9

1F2 c

7.1

34

95.8

1.2

3.0

6C1 c

7.2

36

96.5

1.0

2.5

12D1 c

7.3

35

95.8

1.4

2.8

13H3 c

7.2

35

95.9

1.3

2.8

  1. Cells were cultured in MS plus fructose (20 g/L) medium supplemented with L-leucine (10 g/L). The results are the averages of three independent cultivations (the standard deviations were less than 5% of the mean).
  2. a PHA composition was determined by GC.
  3. b R. eutropha PHB-4 expressing phaC1 Ps.
  4. c leucine analog resistant mutants.
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