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Table 3 PHA biosynthesis by R. eutropha PHB-4 expressing phaC1 Ps or leucine analog resistant mutants with the supplementation of 10 g/L leucine

From: Enhanced Incorporation of 3-Hydroxy-4-Methylvalerate Unit into Biosynthetic Polyhydroxyalkanoate Using Leucine as a Precursor

  Dry cell weight (g/L) PHA content (wt%) PHA composition (mol%) a
Strain    3HB 3HV 3H4MV
Parent b 7.2 29 98.8 0.3 0.9
1F2 c 7.1 34 95.8 1.2 3.0
6C1 c 7.2 36 96.5 1.0 2.5
12D1 c 7.3 35 95.8 1.4 2.8
13H3 c 7.2 35 95.9 1.3 2.8
  1. Cells were cultured in MS plus fructose (20 g/L) medium supplemented with L-leucine (10 g/L). The results are the averages of three independent cultivations (the standard deviations were less than 5% of the mean).
  2. a PHA composition was determined by GC.
  3. b R. eutropha PHB-4 expressing phaC1 Ps.
  4. c leucine analog resistant mutants.