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Table 2 PHA biosynthesis by R. eutropha PHB-4 expressing phaC1 Ps or the leucine analog resistant mutants from fructose as the sole carbon source

From: Enhanced Incorporation of 3-Hydroxy-4-Methylvalerate Unit into Biosynthetic Polyhydroxyalkanoate Using Leucine as a Precursor

 

Dry cell weight (g/L)

PHA content (wt%)

PHA composition (mol%) a

Strain

  

3HB

3HV

3H4MV

Parent b

1.6

53

99.1

0.4

0.5

1F2 c

1.5

53

97.6

1.6

0.8

6C1 c

1.7

55

97.6

1.5

0.9

12D1 c

1.6

51

97.4

1.7

0.9

13H3 c

1.7

51

97.4

1.7

0.9

  1. Cells were cultured in MS plus fructose (20 g/L) medium. The results are the averages of three independent cultivations (the standard deviations were less than 5% of the mean).
  2. a PHA composition was determined by GC.
  3. b R. eutropha PHB-4 expressing phaC1 Ps.
  4. c leucine analog resistant mutants.