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Table 2 PHA biosynthesis by R. eutropha PHB-4 expressing phaC1 Ps or the leucine analog resistant mutants from fructose as the sole carbon source

From: Enhanced Incorporation of 3-Hydroxy-4-Methylvalerate Unit into Biosynthetic Polyhydroxyalkanoate Using Leucine as a Precursor

  Dry cell weight (g/L) PHA content (wt%) PHA composition (mol%) a
Strain    3HB 3HV 3H4MV
Parent b 1.6 53 99.1 0.4 0.5
1F2 c 1.5 53 97.6 1.6 0.8
6C1 c 1.7 55 97.6 1.5 0.9
12D1 c 1.6 51 97.4 1.7 0.9
13H3 c 1.7 51 97.4 1.7 0.9
  1. Cells were cultured in MS plus fructose (20 g/L) medium. The results are the averages of three independent cultivations (the standard deviations were less than 5% of the mean).
  2. a PHA composition was determined by GC.
  3. b R. eutropha PHB-4 expressing phaC1 Ps.
  4. c leucine analog resistant mutants.