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Table 1 PHA biosynthesis by R. eutropha PHB-4 expressing phaC1 Ps with the supplementation of various amino acids

From: Enhanced Incorporation of 3-Hydroxy-4-Methylvalerate Unit into Biosynthetic Polyhydroxyalkanoate Using Leucine as a Precursor

  Dry cell weight (g/L) PHA content (wt%) PHA composition (mol%) a
Amino acid    3HB 3HV 3H4MV
none 1.6 53 99.1 0.4 0.5
L-Val trace b - - - -
L-Leu 7.2 29 98.8 0.3 0.9
L-Ile 5.7 17 92.3 7.7 0
L-Thr 7.6 43 99.3 0.4 0.3
D-Leu trace b - - - -
  1. Cells were cultured in MS plus fructose (20 g/L) medium supplemented with each amino acid (10 g/L). The results are the average of three independent cultivations (the standard deviations were less than 5% of the mean).
  2. a PHA composition was determined by GC.
  3. b less than 0.1 g/L.