Enhanced Incorporation of 3-Hydroxy-4-Methylvalerate Unit into Biosynthetic Polyhydroxyalkanoate Using Leucine as a Precursor

AMB Express

Table 1 PHA biosynthesis by R. eutropha PHB^-4 expressing phaC1 _Ps with the supplementation of various amino acids

Cells were cultured in MS plus fructose (20 g/L) medium supplemented with each amino acid (10 g/L). The results are the average of three independent cultivations (the standard deviations were less than 5% of the mean).
^a PHA composition was determined by GC.
^b less than 0.1 g/L.