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Table 7 T-DNA integration patterns observed in transformants obtained under the indicated co-cultivation conditions

From: Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Integration pattern

Number of transformants

 

1:5000

on platea

1:104 on

platea

1:50 in

liquida

1 copy

10

(50.0%)

18

(30.0%)

16

(40.0%)

1 copy

+ vector backbone

3

(15.0%)

14

(23.3%)

5

(12.5%)

multiple copies at

single locus

5

(25.0%)

3

(5.0%)

8

(20.0%)

multiple copies at

single locus + vector

backbone

1

(5.0%)

19

(31.7%)

7

(17.5%)

multiple copies at

different loci

1

(5.0%)

1

(1.7%)

1

(2.5%)

multiple copies at

different loci + vector backbone

0

(0%)

5

(8.3%)

3

(7.5%)

Number of

transformants analyzed

20

(100%)

60

(100%)

40

(100%)

  1. a, the ratio of A. aculeatus conidia to A. tumefaciens cells.