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Table 7 T-DNA integration patterns observed in transformants obtained under the indicated co-cultivation conditions

From: Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Integration pattern Number of transformants
  1:5000
on platea
1:104 on
platea
1:50 in
liquida
1 copy 10
(50.0%)
18
(30.0%)
16
(40.0%)
1 copy
+ vector backbone
3
(15.0%)
14
(23.3%)
5
(12.5%)
multiple copies at
single locus
5
(25.0%)
3
(5.0%)
8
(20.0%)
multiple copies at
single locus + vector
backbone
1
(5.0%)
19
(31.7%)
7
(17.5%)
multiple copies at
different loci
1
(5.0%)
1
(1.7%)
1
(2.5%)
multiple copies at
different loci + vector backbone
0
(0%)
5
(8.3%)
3
(7.5%)
Number of
transformants analyzed
20
(100%)
60
(100%)
40
(100%)
  1. a, the ratio of A. aculeatus conidia to A. tumefaciens cells.