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Table 6 Optimization of transformation conditions for A. aculeatus pyrG mutant

From: Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Strain Germination
time (h)
Uridine
Concentration in IM
Co-cultivation time and Mean of transformants ± SD
/107 conidia/100 ml IM
 
    24 h 36 h 48 h 60 h 72 h
WT 0 - 23 ± 20 132 ± 82 217 ± 141 292 ± 211 148 ± 101
  0 0.01% 81 ± 72 73 ± 36 116 ± 52 147 ± 64 82 ± 45
  0 0.2% 10 ± 12 193 ± 98 174 ± 106 187 ± 130 131 ± 118
pyrG - 0 0.01% 0 50 ± 38 24 ± 33 15 ± 12a 16 ± 13
  0 0.2% 0 66 ± 71 58 ± 95 135 ± 155a 33 ± 54
  24 0.01% 91 ± 66 122 ± 62 50 ± 49 140 ± 82a 50 ± 60
  24 0.2% 38 ± 24 26 ± 10 23 ± 11 2 ± 5a 12 ± 18
  1. WT, the A. aculeatus wild-type strain; n = 3 except for a (n = 2)