Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

AMB Express

Table 6 Optimization of transformation conditions for A. aculeatus pyrG mutant

Strain	Germination time (h)	Uridine Concentration in IM	Co-cultivation time and Mean of transformants ± SD /10⁷ conidia/100 ml IM
			24 h	36 h	48 h	60 h	72 h
WT	0	-	23 ± 20	132 ± 82	217 ± 141	292 ± 211	148 ± 101
	0	0.01%	81 ± 72	73 ± 36	116 ± 52	147 ± 64	82 ± 45
	0	0.2%	10 ± 12	193 ± 98	174 ± 106	187 ± 130	131 ± 118
pyrG ^-	0	0.01%	0	50 ± 38	24 ± 33	15 ± 12^a	16 ± 13
	0	0.2%	0	66 ± 71	58 ± 95	135 ± 155^a	33 ± 54
	24	0.01%	91 ± 66	122 ± 62	50 ± 49	140 ± 82^a	50 ± 60
	24	0.2%	38 ± 24	26 ± 10	23 ± 11	2 ± 5^a	12 ± 18