Table 5 Optimization of AMT conditions in 100 ml of liquid IM
Number of conidia | Number of Agrobacterium cells | Ratio of conidia: Agrobacterium | Mean of transformants ± SD/107 conidia/100 ml IM (na ≥3) |  | |||
|---|---|---|---|---|---|---|---|
| Â | Â | Â | 24 h | 36 h | 48 h | 60 h | 72 h |
1 ×107 | 5 ×106 | 1: 0.5 | 1 ± 4 | 16 ± 15 | 40 ± 27 | 41 ± 23 | 25 ± 16 |
1 ×107 | 5 ×107 | 1: 5 | 16 ± 27 | 60 ± 29 | 92 ± 88 | 55 ± 16 | 41 ± 26 |
1 ×107 | 5 ×108 | 1: 50 | 23 ± 20 | 132 ± 82 | 217 ± 141 | 292 ± 211 | 148 ± 101 |
1 ×107 | 5 ×109 | 1: 500 | 5 ± 8 | 3 ± 5 | 11 ± 12 | 10 ± 8 | 11 ± 14 |