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Table 5 Optimization of AMT conditions in 100 ml of liquid IM

From: Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Number of
conidia
Number of
Agrobacterium
cells
Ratio of conidia:
Agrobacterium
Mean of transformants ± SD/107
conidia/100 ml IM (na ≥3)
 
    24 h 36 h 48 h 60 h 72 h
1 ×107 5 ×106 1: 0.5 1 ± 4 16 ± 15 40 ± 27 41 ± 23 25 ± 16
1 ×107 5 ×107 1: 5 16 ± 27 60 ± 29 92 ± 88 55 ± 16 41 ± 26
1 ×107 5 ×108 1: 50 23 ± 20 132 ± 82 217 ± 141 292 ± 211 148 ± 101
1 ×107 5 ×109 1: 500 5 ± 8 3 ± 5 11 ± 12 10 ± 8 11 ± 14
  1. a n, number of independent experiments.