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Table 4 The effect of concentration of the fungal and bacterial cells on AMT

From: Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Number of
conidia
Amount of
Agrobacteria
culture (ml)
Ratio of conidia:
Agrobacterium
Mean of
transformants
± SD/plate
Number of
transformants
/104 conidia
1 ×104 0.1 1:104 30 ± 28 (na = 12) 30 ± 28
2 ×104 0.2 1:104 52 ± 10 (n = 2) 26 ± 5
5 ×104 0.5 1:104 51 ± 13 (n = 2) 10 ± 2
1 ×105 1 1:104 39 ± 14 (n = 6) 3 ± 1
1 ×106 10 1:104 16 ± 9 (n = 3) < 1
  1. a n, number of independent experiments.