Skip to main content
AMB Express

Table 4 The effect of concentration of the fungal and bacterial cells on AMT

From: Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Number of

conidia

Amount of

Agrobacteria

culture (ml)

Ratio of conidia:

Agrobacterium

Mean of

transformants

± SD/plate

Number of

transformants

/104 conidia

1 ×104

0.1

1:104

30 ± 28 (na = 12)

30 ± 28

2 ×104

0.2

1:104

52 ± 10 (n = 2)

26 ± 5

5 ×104

0.5

1:104

51 ± 13 (n = 2)

10 ± 2

1 ×105

1

1:104

39 ± 14 (n = 6)

3 ± 1

1 ×106

10

1:104

16 ± 9 (n = 3)

< 1

  1. a n, number of independent experiments.
Back to article page