AMB Express

Table 2 Thermal settings for TAIL-PCR

From: Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Reaction and cycle	Thermal settings
Primary
1	93°C, 1 min.; 95°C, 1 min.
5	98°C, 30 sec.; 62°C, 15 sec.; 72°C, 3 min.
	98°C, 30 sec.; 25°C, 3 min.;
1	ramping to 72°C, over 3 min.; 72°C, 3 min.
	98°C, 10 sec.; 68°C, 15 sec.; 72°C, 3 min.;
15	98°C, 10 sec.; 68°C, 15 sec.; 72°C, 3 min.;
	98°C, 10 sec.; 44°C, 15 sec.; 72°C, 3 min.
1	72°C, 5 min.
Secondary
1	93°C, 2 min.
	98°C, 10 sec.; 64°C, 15 sec.; 72°C, 3 min.;
12	98°C, 10 sec.; 64°C, 15 sec.; 72°C, 3 min.;
	98°C, 10 sec.; 44°C, 15 sec.; 72°C, 3 min.
1	72°C, 5 min.
Tertiary
1	93°C, 2 min.
	98°C, 10 sec.; 68°C, 15 sec.; 72°C, 3 min.;
12	98°C, 10 sec.; 68°C, 15 sec.; 72°C, 3 min.;
	98°C, 10 sec.; 44°C, 15 sec.; 72°C, 3 min.
1	72°C, 5 min.

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