Table 3 Purification of biodegradable plastic-degrading enzyme from P. antarctica JCM10317
From: Phyllosphere yeasts rapidly break down biodegradable plastics
Source of fraction | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) |
|---|---|---|---|---|
Culture filtrate | 23.306 | 28.96 | 1.24 | 100.0 |
Ammonium sulfate precipitation | 0.422 | 3.42 | 8.11 | 11.83 |
Ion exchange chromatography | 0.014 | 2.73 | 198.35 | 9.44 |
Gel filtration | 0.003 | 1.34 | 398.16 | 4.61 |