Figure 5

SDS-PAGE of biodegradable plastic-degrading enzyme (PaE) from Pseudozyma antarctica JCM 10317 and a commercially available lipase, CALB-L. (A) Purification of PaE. M Molecular mass standards; 1 Ammoniun sulfate precipitate; Fractions of 2 passed through the DEAE-Sepharose column; 3 eluated from SP-Sepharose column; 4 Gel filtration. SDS-PAGE was performed using 12% gel and detected by silver staining (B) Purified PaE and CALB-L. M Molecular mass standards; 1 purified PaE (0.25 μg); 2 CALB-L (25 μg). SDS-PAGE was performed using 14.1% gel and detected by CBB. The black arrow in the PaE lane shows the PaE isolated from the culture medium. The black arrow in the CALB-L lane indicates lipase B. The white arrows indicate impurities.