Skip to main content

Table 2 Purification of recombinant NADP-dependent meso- DAPDH from E. coli

From: Highly stable meso-diaminopimelate dehydrogenase from an Ureibacillus thermosphaericus strain A1 isolated from a Japanese compost: purification, characterization and sequencing

Purification step b Total
protein
Total
activity
Specific
activity
Yield
  mg units units/mg %
Crude extract 250 796 3.18 100
Heat-treatment 198 694 3.51 87.1
Chelating Sepharose™ Fast Flow column 54.6 423 7.75 53.1
  1. b) The cells were obtained from a 500 ml-medium