Table 2 Purification of recombinant NADP-dependent meso- DAPDH from E. coli
Purification step b | Total protein | Total activity | Specific activity | Yield |
|---|---|---|---|---|
mg | units | units/mg | % | |
Crude extract | 250 | 796 | 3.18 | 100 |
Heat-treatment | 198 | 694 | 3.51 | 87.1 |
Chelating Sepharose™ Fast Flow column | 54.6 | 423 | 7.75 | 53.1 |