Highly stable meso-diaminopimelate dehydrogenase from an Ureibacillus thermosphaericus strain A1 isolated from a Japanese compost: purification, characterization and sequencing

AMB Express

Table 1 Purification of NADP-dependent meso- DAPDH from U. thermosphaeicus

Purification step ^a	Total protein	Total activity	Specific activity	Yield
	mg	units	units/mg	%
Crude extract	264	46.6	0.177	100
80% Ammonium sulfate	162	44.5	0.274	95.5
Butyl Sepharose™ 4 Fast Flow column	15.1	15.7	1.04	33.7
DEAE-Toyopearl 650M column	2.90	5.01	1.73	10.8
Preparative slab PAGE	0.264	2.19	8.28	4.69