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Table 1 Purification of NADP-dependent meso- DAPDH from U. thermosphaeicus

From: Highly stable meso-diaminopimelate dehydrogenase from an Ureibacillus thermosphaericus strain A1 isolated from a Japanese compost: purification, characterization and sequencing

Purification step a Total
protein
Total
activity
Specific
activity
Yield
  mg units units/mg %
Crude extract 264 46.6 0.177 100
80% Ammonium sulfate 162 44.5 0.274 95.5
Butyl Sepharose™ 4 Fast Flow column 15.1 15.7 1.04 33.7
DEAE-Toyopearl 650M column 2.90 5.01 1.73 10.8
Preparative slab PAGE 0.264 2.19 8.28 4.69
  1. a) The cells were obtained from a 500 ml-medium.