Pyrosequencing of 16S rRNA gene amplicons to study the microbiota in the gastrointestinal tract of carp (Cyprinus carpio L.)

AMB Express

Table 1 Primer specifications

Primer	Target	Sequence (5'-3')	Reference
Pla46 F	Planctomycetales	GGATTAGGCATGCAAGTC	Neef et al. 1998
616 F	Most bacteria	AGAGTTTGATYMTGGCTCAG	Juretschko et al. 1998
EubI R	Most bacteria	GCTGCCTCCCGTAGGAGT	Amann et al. 1990
EubII R	Planctomycetales	GCAGCCACCCGTAGGTGT	Daims et al. 1999
EubIII R	Verrucomicrobiales	GCTGCCACCCGTAGGTGT	Daims et al. 1999