A mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw

AMB Express

Table 2 S. cerevisiae strains and plasmids used in this study.

Strains and Plasmids	Relevant Genotype	Reference
Plasmids
YIpOB8	URA3 TDH3p-XYL1-ADH1t, PGK1p-XYL2-PGK1t	(Bengtsson et al. 2009)
YIplac128	LEU2	(Gietz and Sugino 1988)
YIpDR1	YIplac128 TDH3p-GXF1-CYC1t	(Runquist et al. 2009)
YIpDR7	pOB8 XR N272D	(Runquist et al. 2010a)
S. cerevisiae strains
TMB 3043	CEN.PK 2-1C Δgre3, his3::PGK1p-XKS1-PGK1t, TAL1::PGK1p-TAL1-PGK1t, TKL1::PGK1p-TKL1-PGK1t, RKI1::PGK1p-RKI1-PGK1t, RPE1::PGK1p-RPE1-PGK1t, leu2, ura3	(Karhumaa et al. 2005)
TMB 3043-Gxf1	TMB 3043, leu2::YIpDR1, ura3	(Runquist et al. 2009)
TMB 3422	TMB 3043, leu2::YIplac128, ura3::YIpDR7	(Runquist et al. 2010a)
TMB 3424	TMB 3043, leu2::YIplac128, ura3:: YIpOB8	(Runquist et al. 2010a)
TMB 3425	TMB 3043, leu2::YIpDR1, ura3::YIpDR7	This work
TMB 3426	TMB 3043, leu2::YIpDR1, ura3::YIpOB8	This work