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Table 2 S. cerevisiae strains and plasmids used in this study.

From: A mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw

Strains and Plasmids

Relevant Genotype

Reference

Plasmids

  

YIpOB8

URA3 TDH3p-XYL1-ADH1t, PGK1p-XYL2-PGK1t

(Bengtsson et al. 2009)

YIplac128

LEU2

(Gietz and Sugino 1988)

YIpDR1

YIplac128 TDH3p-GXF1-CYC1t

(Runquist et al. 2009)

YIpDR7

pOB8 XR N272D

(Runquist et al. 2010a)

S. cerevisiae strains

  

TMB 3043

CEN.PK 2-1C Δgre3, his3::PGK1p-XKS1-PGK1t, TAL1::PGK1p-TAL1-PGK1t, TKL1::PGK1p-TKL1-PGK1t, RKI1::PGK1p-RKI1-PGK1t, RPE1::PGK1p-RPE1-PGK1t, leu2, ura3

(Karhumaa et al. 2005)

TMB 3043-Gxf1

TMB 3043, leu2::YIpDR1, ura3

(Runquist et al. 2009)

TMB 3422

TMB 3043, leu2::YIplac128, ura3::YIpDR7

(Runquist et al. 2010a)

TMB 3424

TMB 3043, leu2::YIplac128, ura3:: YIpOB8

(Runquist et al. 2010a)

TMB 3425

TMB 3043, leu2::YIpDR1, ura3::YIpDR7

This work

TMB 3426

TMB 3043, leu2::YIpDR1, ura3::YIpOB8

This work