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Table 2 Primers and peptide sequences used in this work

From: Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri

Primer name

Primer sequence a

yqhDF (Forward)

5'-CATG CCATGG ACAACAACTTTAATCTGCACACC-3'

yqhDR (Reverse)

5'-CCG CTCGAG TTAGCGGGCGGCTTC-3'

PorfXF (Forward)

SppIP

5'-TGAAAATTGATATTAGCG-3'

MAGNSSNFIHKIKQIFTHR

  1. a The restriction sites in the primers Nco I (forward) and Xho I (reverse) have been underlined