Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri

AMB Express

Table 1 Bacterial strains and plasmid vectors used in this work

Strain or plasmid	Description	Source or reference
E. coli DH5α	Cloning host for TA vector	Invitrogen, USA
E. coli EC1000	Cloning host for pSIP411	Dr Jan Kok, University of Groningen, Netherlands
RBC- TA vector	TA cloning vector	RBC Bioscience Corp., Taiwan
pSIP411	E. coli-lactobacillus shuttle expression vector	Sørvig et al. (2005)
L. reuteri ATCC55730	Host	Biogaia, Sweden
L. reuteri HR2	L. reuteri with yqhD	This study
E. coli K-12 MG1655	Source of yqh D gene	Prof. Takashi Horiuchi, National Institute for Basic Biology, Japan.
pHR1	TA vector with yqhD	This study
pHR2	pSIP411 with yqh D	This study