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Table 1 Bacterial strains and plasmid vectors used in this work

From: Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri

Strain or plasmid


Source or reference

E. coli DH5α

Cloning host for TA vector

Invitrogen, USA

E. coli EC1000

Cloning host for pSIP411

Dr Jan Kok, University of Groningen, Netherlands

RBC- TA vector

TA cloning vector

RBC Bioscience Corp., Taiwan


E. coli-lactobacillus shuttle expression vector

Sørvig et al. (2005)

L. reuteri ATCC55730


Biogaia, Sweden

L. reuteri HR2

L. reuteri with yqhD

This study

E. coli K-12 MG1655

Source of yqh D gene

Prof. Takashi Horiuchi, National Institute for Basic Biology, Japan.


TA vector with yqhD

This study


pSIP411 with yqh D

This study