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Table 1 Bacterial strains and plasmid vectors used in this work

From: Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri

Strain or plasmid Description Source or reference
E. coli DH5α Cloning host for TA vector Invitrogen, USA
E. coli EC1000 Cloning host for pSIP411 Dr Jan Kok, University of Groningen, Netherlands
RBC- TA vector TA cloning vector RBC Bioscience Corp., Taiwan
pSIP411 E. coli-lactobacillus shuttle expression vector Sørvig et al. (2005)
L. reuteri ATCC55730 Host Biogaia, Sweden
L. reuteri HR2 L. reuteri with yqhD This study
E. coli K-12 MG1655 Source of yqh D gene Prof. Takashi Horiuchi, National Institute for Basic Biology, Japan.
pHR1 TA vector with yqhD This study
pHR2 pSIP411 with yqh D This study