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Table 3 Apparent kinetic parameters of purified Mro APO

From: High-yield production of aromatic peroxygenase by the agaric fungus Marasmius rotula

Substrate pH K m
(mM)
k cat
(s -1 )
k cat /K m
(s-1 M-1)
ABTS 4.5 0.071 25 3,53*105
DMP 5.5 0.133 70 5.29*105
Benzyl alcohol 5.5 0.118 62 5.29*105
Veratryl alcohol 5.5 0.279 49 1.76*105
Naphthalene 5.5 0.791 33 4.25*104
H2O2 5.5 3.14 76 2.42*104
  1. Reactions were performed in sodium phosphate/citrate buffer (pH 4.5 or 5.5) in the presence of 2 mM H2O2 at 25°C. The Km for the co-substrate H2O2 was determined in the presence of 5 mM veratryl alcohol. The data are means of three parallel experiments with standard deviation < 5%.