Table 1 Exemplary table for the purification of Mro APO by fast protein liquid chromatography (FPLC)
From: High-yield production of aromatic peroxygenase by the agaric fungus Marasmius rotula
Steps | Total Activity (Units) | Total Protein (mg) | Specific Activity (U mg-1) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Culture fluid | 11,700 | 730 | 16 | 100 | 1 |
Ultrafiltration step | 11,520 | 415 | 28 | 98 | 1.7 |
Q Sepharose FF | 10,413 | 218 | 48 | 89 | 3.0 |
Mono Q | 6,235 | 118 | 53 | 53 | 3.3 |
Source 15Q | 1,457 | 22 | 88 | 17 | 5.5 |
SEC | 768 | 9 | 90 | 7 | 5.6 |
Mono Q | 75 | 0.98 | 76 | 0.7 | 4.8 |