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Table 1 Exemplary table for the purification of Mro APO by fast protein liquid chromatography (FPLC)

From: High-yield production of aromatic peroxygenase by the agaric fungus Marasmius rotula

Steps Total Activity
(Units)
Total Protein
(mg)
Specific Activity
(U mg-1)
Yield
(%)
Purification
(fold)
Culture fluid 11,700 730 16 100 1
Ultrafiltration step 11,520 415 28 98 1.7
Q Sepharose FF 10,413 218 48 89 3.0
Mono Q 6,235 118 53 53 3.3
Source 15Q 1,457 22 88 17 5.5
SEC 768 9 90 7 5.6
Mono Q 75 0.98 76 0.7 4.8
  1. Enzyme activities are based on the oxidation of veratryl alcohol to veratrylaldehyde at pH 5.5. The purification processes started with 468 mL culture fluid from the bioreactor fermentation (volume activity 25 U mL-1) corresponding to a total of 11,700 Units.