Multiple sequence alignment of AS-protease gene sequence from metagenome. Proteases used for alignment are S. baltica, peptidase S8 and S53 subtilisin kexin sedolisin [Shewanella baltica OS185] (YP_001367387.1); S. violacea, extracellular alkaline serine protease precursor, putative [Shewanella violacea DSS12] (YP_003556880.1); S. denitrificans, peptidase S8 and S53, subtilisin, kexin, sedolisin [Shewanella denitrificans OS217] (YP_562027.1). Pseudoalteromonas, extracellular alkaline serine protease 2 [Pseudoalteromonas sp. AS-11]. The AS-protease sequence identified from metagenome is indicated by arrows in the left. Conserved residues are letters in dark blue background. Catalytic residues are boxed in red outline.