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Table1 List of mutagenic primers used in site-directed mutagenesis experiments

From: PEGylating a bacteriophage endolysin inhibits its bactericidal activity

Cpl-1 mutant

Forward mutagenic primer

Reverse mutagenic primer

Cpl-1C45S

5'-CGA CCT ATT TAA ACC CTA GCT TGT CTG CTC AAG TGG AGC AGT CAA ACC C-3'

5'-GGG TTT GAC TGC TCC ACT TGA GCA GAC AAG CT A GGG TTT AAA TAG GTC G-3'

Cpl1C45S;Q85C

5'-GTT TTT CCT TGA CAA CGT GCC TAT GTG C GT TAA ATA CCT TGT ATT GGA CTA CG-3'

5'-CGT AGT CCA ATA CAA GGT ATT TAA CGC A CA TAG GCA CGT TGT CAA GGA AAA AC-3'

Cpl1C45S;D194C

5'-GTT AGA CGA TGA AGA AGA CTG CAA GCC AAA GAC CGC TGG A-3'

5'-TCC AGC GGT CTT TGG CTT GCA GTC TTC TTC ATC GTC TAA C-3'

Cpl1C45S;N214C

5'-GGG TGG TGG TTC AGA CGA TG C AAT GGC AGT TTC CCT TA-3'

5'-TAA GGG AAA CTG CCA TTG CA T CGT CTG AAC CAC CAC CC-3'

Cpl-1C45S;G216C

5'-GTG GTG GTT CAG ACG AAA CAA TTG CAG TTT CCC TT-3'

5'-AAG GGA AAC TGC A AT TGT TTC GTC TGA ACC ACC AC-3'

Cpl-1C45S;D256C

5'-AAA TGG TAC TAC CTC AAG TG C AAC GGC GCA ATG GCG AC-3'

5'-GTC GCC ATT GCG CCG TTG CA C TTG AGG TAG TAC CAT TT-3'

Cpl-1C45S;S269C

5'-GTT GGG TGC TAG TCG GGT GC G AGT GGT ATT ATA TGG AC-3'

5'-GTC CAT ATA ATA CCA CTC GC A CCC GAC TAG CAC CCA AC-3'

Cpl-1C45S;D324C

5'-ACA CAA ACG GAG AGC TTG CAT G CA ATC CAA GTT TCA CGA AAG-3'

5'-CTT TCG TGA AAC TTG GAT TGC A TG CAA GCT CTC CGT TTG TGT-3'

  1. Mutated positions are underlined.