From: PEGylating a bacteriophage endolysin inhibits its bactericidal activity
Cpl-1 mutant | Forward mutagenic primer | Reverse mutagenic primer |
---|---|---|
Cpl-1C45S | 5'-CGA CCT ATT TAA ACC CTA GCT TGT CTG CTC AAG TGG AGC AGT CAA ACC C-3' | 5'-GGG TTT GAC TGC TCC ACT TGA GCA GAC AAG CT A GGG TTT AAA TAG GTC G-3' |
Cpl1C45S;Q85C | 5'-GTT TTT CCT TGA CAA CGT GCC TAT GTG C GT TAA ATA CCT TGT ATT GGA CTA CG-3' | 5'-CGT AGT CCA ATA CAA GGT ATT TAA CGC A CA TAG GCA CGT TGT CAA GGA AAA AC-3' |
Cpl1C45S;D194C | 5'-GTT AGA CGA TGA AGA AGA CTG CAA GCC AAA GAC CGC TGG A-3' | 5'-TCC AGC GGT CTT TGG CTT GCA GTC TTC TTC ATC GTC TAA C-3' |
Cpl1C45S;N214C | 5'-GGG TGG TGG TTC AGA CGA TG C AAT GGC AGT TTC CCT TA-3' | 5'-TAA GGG AAA CTG CCA TTG CA T CGT CTG AAC CAC CAC CC-3' |
Cpl-1C45S;G216C | 5'-GTG GTG GTT CAG ACG AAA CAA TTG CAG TTT CCC TT-3' | 5'-AAG GGA AAC TGC A AT TGT TTC GTC TGA ACC ACC AC-3' |
Cpl-1C45S;D256C | 5'-AAA TGG TAC TAC CTC AAG TG C AAC GGC GCA ATG GCG AC-3' | 5'-GTC GCC ATT GCG CCG TTG CA C TTG AGG TAG TAC CAT TT-3' |
Cpl-1C45S;S269C | 5'-GTT GGG TGC TAG TCG GGT GC G AGT GGT ATT ATA TGG AC-3' | 5'-GTC CAT ATA ATA CCA CTC GC A CCC GAC TAG CAC CCA AC-3' |
Cpl-1C45S;D324C | 5'-ACA CAA ACG GAG AGC TTG CAT G CA ATC CAA GTT TCA CGA AAG-3' | 5'-CTT TCG TGA AAC TTG GAT TGC A TG CAA GCT CTC CGT TTG TGT-3' |