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Table 3 qRT-PCR probe and reaction descriptions

From: The role of different methanogen groups evaluated by Real-Time qPCR as high-efficiency bioindicators of wet anaerobic co-digestion of organic waste

Target group

Probe name target

Example of regression curve

r 2

PCR efficiency (%)

Acceptable data (%)

Methanosarcina

msar

y = -0.2547x +11.34

0.997

80

75

Methanobacteriaceae

mrtA

y = -0.2691x+12.21

0.995

86

4

Methanocorpusculaceae

mcp

y = -0.2627x+12.38

0.987

83

88

Methanosaetaceae

msa

y = -0.2380x+10.27

0.943

73

52

  1. There is a standard reference curve only for the Methanosarcina and Methanobatecteriaceae, making it possible to establish the gene copies in the extracted DNA. The last column indicates the percentage of determinable sample on the total 25 tested samples.