Target group | Probe name target | Example of regression curve | r 2 | PCR efficiency (%) | Acceptable data (%) |
---|---|---|---|---|---|
Methanosarcina | msar | y = -0.2547x +11.34 | 0.997 | 80 | 75 |
Methanobacteriaceae | mrtA | y = -0.2691x+12.21 | 0.995 | 86 | 4 |
Methanocorpusculaceae | mcp | y = -0.2627x+12.38 | 0.987 | 83 | 88 |
Methanosaetaceae | msa | y = -0.2380x+10.27 | 0.943 | 73 | 52 |