Process development for the elucidation of mycotoxin formation in Alternaria alternata

AMB Express

Table 2 Mycotoxin production in a 2 L bioreactor by A.alternata depending on carbon and nitrogen source at an aeration rate of 0.013 vvm

Carbon source	Nitrogen source	AOH [mg/L]	AME [mg/L]	TA [mg/L]	Process time [h]	BDM [g/L]
^aGlucose	NH₄Cl, NaNO₃	3.49 ± 0.121	1.62 ± 0.142	38.28 ± 1.61	260	3.3 ± 0.1
^aGlucose	Aspartic acid	7.75 ± 0.064	4.81 ± 0.014	36.54 ± 0.81	350	3.49 ± 0.27
^bNa-acetate	NH₄Cl, NaNO₃	6.64 ± 0.010	ND	ND	400	1.98 ± 0.06
Na-acetate	Aspartic acid	3.66	ND	ND	400	2.62

^a Results are mean of 5 replicates ± standard deviation
^b Results are mean of 2 replicates ± standard deviation.
Acetate fermentations were conducted without pH control, glucose fermentations were performed at pH 5.5. Values display maximal detected mycotoxin concentration. Process time gives the earliest time point when the maximum concentration was achieved.
AOH: alternariol; AME: alternariol monomethylether; TA: tenuazonic acid; BDM: biodrymass; ND: below detection limit of < 0.001 mg/L.