Table 1 Effect of different inhibitors on P. excavatus proteases.
Inhibitors | Conc. (mM) | Relative activity (%) | |||||
|---|---|---|---|---|---|---|---|
FI | FII | FIII-1 | FIII-2 | FIII-3 | FIV | ||
Control | 100 | 100 | 100 | 100 | 100 | 100 | |
PMSF | 1 | 0 | 0 | 2 | 4 | 0 | 8 |
TPCK | 0.1 | 100 | 100 | 96 | 100 | 76 | 100 |
Aprotinin | 0.01 | 29 | 95 | 87 | 95 | 26 | 71 |
Leupeptin | 0.1 | 0 | 100 | 98 | 100 | 0 | 73 |
SBTI | 0.01 | 0 | 76 | 81 | 93 | 0 | 0 |
EDTA | 1 | 100 | 100 | 100 | 100 | 100 | 100 |
Chymostatin | 0.1 | 19 | 100 | 76 | 100 | 19 | 90 |
Pepstatin | 1 | 100 | 58 | 60 | 86 | 83 | 75 |