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Table 3 Coenzyme (NADH/NADPH) dependency on ketone reduction using cell-free extracts of Synechococcus

From: Reduction of exogenous ketones depends upon NADPH generated photosynthetically in cells of the cyanobacterium Synechococcus PCC 7942

Substrate Coenzyme Yield of ketone reduction (%)
TFA NADPH 16 (1.00)*
  NADH 3.5 (0.22)*
PF NADPH 12 (1.00)*
  NADH 0.8 (0.07)*
  1. Substrate: 2 mg, 0.1 M potassium phosphate buffer (pH7): 2 mL,
  2. Acetone-dried powder of Synechococcus: 10 mg, NADH or NADPH: 5 mg, 25°C, 44 h
  3. *Values in parentheses are the relative to the yields in the presence of NADPH.
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