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Table 1 Bacterial strains and plasmids used

From: Analysis and application of Bacillus subtilis sortases to anchor recombinant proteins on the cell wall

B. subtilis Description Reference
1012 leuA8 metB5 trpC2 hsdRM1 (Saito et al. 1979)
NDH03 WW02 with srtA gene of Listeria monocytogenes integrated at the lacA locus (Nguyen and Schumann 2006)
NDH20 1012 carrying pNDH26 inserted into the chromosome This work
NDH21 1012 carrying pNDH27 inserted into the chromosome This work
NDH30 1012 yhcS :: neo (NeoR) This work
NDH31 1012 yhcS :: neo, ywpE :: erm (NeoR, ErmR) This work
NDH32 WB800 yhcs:: neo, ywpE::erm (NeoR, ErmR, CmR) This work
SZ59 1012 yhcS :: cat (CmR) This work
SZ60 1012 ywpE :: erm (EmR) This work
WB800 nprE aprE epr bpr mpr :: ble nprB :: bsr Δvpr wprA :: hyg (CmR) (Wu et al. 2002)
WB800N WB800 pB-cat 5-neo-cat 3 (NeoR) This work
Plasmids   
pBluescript II KS   Stratagene
pMUTIN4 Integration vector carrying lacZ and erm (Vagner et al. 1998)
pNDH19 PxylA-amyQ-fnbpB123 (Nguyen and Schumann 2006)
pNDH26 pMUTIN4 carrying 5' end of yhcS This work
pNDH27 pMUTIN4 carrying 5' end of ywpE This work
pNDH33 Expression vector carrying Pgrac and Cm (Phan et al. 2006)
pNDH33-yhcS pNDH33 carrying yhcS (Pgrac-yhcS) This work
pNDH33-ywpE pNDH33 carrying ywpE (Pgrac-ywpE) This work
pNDH33-ywpE-yhcS pNDH33 carrying ywpE-yhcS
(Pgrac-ywpE-yhcS)
This work
pNDH37 Pgra c with signal sequence of amyQ (Phan et al. 2006)
pNDH37-amyQ Pgrac with full length of amyQ (Phan et al. 2006)
pNDH88 pHT01 with amyQ This work
pNDH89 yhcR123 translationally fused to amyQ This work
pNDH90 yfkN123 translationally fused to amyQ This work
pHT01 Expression vector carrying Pgrac and Cm (Nguyen et al. 2007)