Table 1 Bacterial strains and plasmids used

1012

leuA8 metB5 trpC2 hsdRM1

(Saito et al. 1979)

NDH03

WW02 with srtA gene of Listeria monocytogenes integrated at the lacA locus

(Nguyen and Schumann 2006)

NDH20

1012 carrying pNDH26 inserted into the chromosome

This work

NDH21

1012 carrying pNDH27 inserted into the chromosome

This work

NDH30

1012 yhcS :: neo (Neo^R)

This work

NDH31

1012 yhcS :: neo, ywpE :: erm (Neo^R, Erm^R)

This work

NDH32

WB800 yhcs:: neo, ywpE::erm (Neo^R, Erm^R, Cm^R)

This work

SZ59

1012 yhcS :: cat (Cm^R)

This work

SZ60

1012 ywpE :: erm (Em^R)

This work

WB800

nprE aprE epr bpr mpr :: ble nprB :: bsr Δvpr wprA :: hyg (Cm^R)

(Wu et al. 2002)

WB800N

WB800 pB-cat 5-neo-cat 3 (Neo^R)

This work

Plasmids

pBluescript II KS

Stratagene

pMUTIN4

Integration vector carrying lacZ and erm

(Vagner et al. 1998)

pNDH19

PxylA-amyQ-fnbpB123

(Nguyen and Schumann 2006)

pNDH26

pMUTIN4 carrying 5' end of yhcS

This work

pNDH27

pMUTIN4 carrying 5' end of ywpE

This work

pNDH33

Expression vector carrying Pgrac and Cm

(Phan et al. 2006)

pNDH33-yhcS

pNDH33 carrying yhcS (Pgrac-yhcS)

This work

pNDH33-ywpE

pNDH33 carrying ywpE (Pgrac-ywpE)

This work

pNDH33-ywpE-yhcS

pNDH33 carrying ywpE-yhcS

(Pgrac-ywpE-yhcS)

This work

pNDH37

Pgra c with signal sequence of amyQ

(Phan et al. 2006)

pNDH37-amyQ

Pgrac with full length of amyQ

(Phan et al. 2006)

pNDH88

pHT01 with amyQ

This work

pNDH89

yhcR123 translationally fused to amyQ

This work

pNDH90

yfkN123 translationally fused to amyQ

This work

pHT01

Expression vector carrying Pgrac and Cm

(Nguyen et al. 2007)