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AMB Express

Table 3 Recovery factor percentage

From: A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control

soybean seeds (*)

R 4 (**) [cfu.seed -1 ]

R 0 (**) [cfu.seed -1 ]

RFP 4 hours at 20°C (***)

4870 sample 1 (AD)

1.90 105

1.47 106

12.9

4870 sample 2 (AD)

1.52 105

1.12 106

13.6

4870 sample 3 (AD)

1.53 105

9.82 105

15.6

3810-pb sample 1 (AD)

1.40 105

2.65 106

5.28

3810-pb sample 1 (PD)

4.00 105

2.65 106

15.1

3810-pb sample 2 (AD)

1.20 105

2.50 106

4.8

3810-pb sample 2 (PD)

3.90 105

2.50 106

15.6

  1. Bacterial direct count and RFP at 20°C after 4 hours of inoculation with B. japonicum strain 61A273 into two commercial soybean seeds
  2. R4: cfu.seed-1 4 hours after inoculation; R0: cfu.seed-1 theoretically provided by inoculant; RFP: Recovery Factor (%).
  3. (*) cv. 4870: 6,200 seeds.kg-1 and cv. 3810-pb: 6,000 seeds.kg-1
  4. (**) average of three determinations
  5. (***) bacterial counting in inoculant used for cv. 4870: sample 1 (3.04 109 cfu.ml-1); sample 2 (2.32.109 cfu.ml-1); sample 3 (2.03.109 cfu.ml-1).bacterial counting in inoculant used for cv. 3810-pb: sample 1 (5.3 109 cfu.ml-1); 3810-prebásica sample 2 (5.0 109 cfu.ml-1).
  6. (AD) treated with a dose of 3 ml.kg-1 seed of the inoculant (agronomic dose)
  7. (PD) treated with a dose of 3 ml.kg-1 seed of inoculant and 0.3 ml.kg-1of sucrose 80% solution
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