soybean seeds
(*)
|
R
4
(**)
[cfu.seed
-1
]
|
R
0
(**)
[cfu.seed
-1
]
|
RFP 4 hours at 20°C
(***)
|
---|
4870 sample 1 (AD)
|
1.90 105
|
1.47 106
|
12.9
|
4870 sample 2 (AD)
|
1.52 105
|
1.12 106
|
13.6
|
4870 sample 3 (AD)
|
1.53 105
|
9.82 105
|
15.6
|
3810-pb sample 1 (AD)
|
1.40 105
|
2.65 106
|
5.28
|
3810-pb sample 1 (PD)
|
4.00 105
|
2.65 106
|
15.1
|
3810-pb sample 2 (AD)
|
1.20 105
|
2.50 106
|
4.8
|
3810-pb sample 2 (PD)
|
3.90 105
|
2.50 106
|
15.6
|
- Bacterial direct count and RFP at 20°C after 4 hours of inoculation with B. japonicum strain 61A273 into two commercial soybean seeds
- R4: cfu.seed-1 4 hours after inoculation; R0: cfu.seed-1 theoretically provided by inoculant; RFP: Recovery Factor (%).
- (*) cv. 4870: 6,200 seeds.kg-1 and cv. 3810-pb: 6,000 seeds.kg-1
- (**) average of three determinations
- (***) bacterial counting in inoculant used for cv. 4870: sample 1 (3.04 109 cfu.ml-1); sample 2 (2.32.109 cfu.ml-1); sample 3 (2.03.109 cfu.ml-1).bacterial counting in inoculant used for cv. 3810-pb: sample 1 (5.3 109 cfu.ml-1); 3810-prebásica sample 2 (5.0 109 cfu.ml-1).
-
(AD) treated with a dose of 3 ml.kg-1 seed of the inoculant (agronomic dose)
-
(PD) treated with a dose of 3 ml.kg-1 seed of inoculant and 0.3 ml.kg-1of sucrose 80% solution