Skip to main content
AMB Express

Table 3 Recovery factor percentage

From: A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control

soybean seeds (*) R 4 (**) [cfu.seed -1 ] R 0 (**) [cfu.seed -1 ] RFP 4 hours at 20°C (***)
4870 sample 1 (AD) 1.90 105 1.47 106 12.9
4870 sample 2 (AD) 1.52 105 1.12 106 13.6
4870 sample 3 (AD) 1.53 105 9.82 105 15.6
3810-pb sample 1 (AD) 1.40 105 2.65 106 5.28
3810-pb sample 1 (PD) 4.00 105 2.65 106 15.1
3810-pb sample 2 (AD) 1.20 105 2.50 106 4.8
3810-pb sample 2 (PD) 3.90 105 2.50 106 15.6
  1. Bacterial direct count and RFP at 20°C after 4 hours of inoculation with B. japonicum strain 61A273 into two commercial soybean seeds
  2. R4: cfu.seed-1 4 hours after inoculation; R0: cfu.seed-1 theoretically provided by inoculant; RFP: Recovery Factor (%).
  3. (*) cv. 4870: 6,200 seeds.kg-1 and cv. 3810-pb: 6,000 seeds.kg-1
  4. (**) average of three determinations
  5. (***) bacterial counting in inoculant used for cv. 4870: sample 1 (3.04 109 cfu.ml-1); sample 2 (2.32.109 cfu.ml-1); sample 3 (2.03.109 cfu.ml-1).bacterial counting in inoculant used for cv. 3810-pb: sample 1 (5.3 109 cfu.ml-1); 3810-prebásica sample 2 (5.0 109 cfu.ml-1).
  6. (AD) treated with a dose of 3 ml.kg-1 seed of the inoculant (agronomic dose)
  7. (PD) treated with a dose of 3 ml.kg-1 seed of inoculant and 0.3 ml.kg-1of sucrose 80% solution
Back to article page