Table 3 Production and distribution of inulinase in static batch cultures of K. marxianus DMKU 3-1042 in YP medium with 2% various carbon substrates
Medium | Temperature (°C) | Total inulinase activity (U mg of cell dry wt -1 ) a | Inulinase activity (U mg of cell dry wt -1 ) a | |
|---|---|---|---|---|
Supernatant (%) | Cell (%) | |||
YPD | 30 | 600 | 310 (52) | 290 (48) |
45 | 1140 | 880 (77) | 260 (23) | |
YPSuc | 30 | 650 | 310 (48) | 340 (52) |
45 | 1290 | 1000 (78) | 290 (22) | |
YPInu | 30 | 1920 | 1000 (52) | 920 (48) |
45 | 1390 | 820 (59) | 570 (41) | |
YPDSuc | 30 | 640 | 410 (64) | 230 (36) |
45 | 1570 | 1300(83) | 270 (17) | |
YPDInu | 30 | 590 | 320 (54) | 270 (46) |
45 | 1230 | 960 (78) | 270 (22) | |