Effect of 2-DOG on fructose uptake. Cells grown in YPD medium at 30°C for 18 h were inoculated into sequential batch culture, which was conducted in 300-ml Erlenmeyer flask containing 100 ml of YP medium containing 2% fructose (YPFrt), 2% sucrose (YPSuc), 2% raffinose (YPRaf) or 2% inulin (YPInu) supplemented with 0.01% 2-DOG. Cultivation was continued further at 30°C (a) or 45°C (b) under a static condition as time indicated. Initial OD660 was adjusted to 1.0. Bars represent the ±SD for three independent experiments.