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Table 2 Purification of alliinase from E. adhaerens FERM P-19486

From: Alliinase from Ensifer adhaerens and Its Use for Generation of Fungicidal Activity

Purification step Protein
(mg)
Total activity
(units)
Sp. Act
(units/mg)
Yield
(%)
Purification
(fold)
Crude extract 209.81 73.0 0.3 100 1
DEAE-cellulose 9.60 30.0 3.1 41.1 10
Phenyl-sepharose 1.94 22.4 11.5 30.7 38
Aminohexyl-sepharose 0.29 7.5 25.9 10.3 86
Mono Q 0.04 4.1 102.5 5.6 341
TSK-GEL 0.01 0.9 90.0 1.2 300