From: Alliinase from Ensifer adhaerens and Its Use for Generation of Fungicidal Activity
Purification step | Protein (mg) | Total activity (units) | Sp. Act (units/mg) | Yield (%) | Purification (fold) |
---|---|---|---|---|---|
Crude extract | 209.81 | 73.0 | 0.3 | 100 | 1 |
DEAE-cellulose | 9.60 | 30.0 | 3.1 | 41.1 | 10 |
Phenyl-sepharose | 1.94 | 22.4 | 11.5 | 30.7 | 38 |
Aminohexyl-sepharose | 0.29 | 7.5 | 25.9 | 10.3 | 86 |
Mono Q | 0.04 | 4.1 | 102.5 | 5.6 | 341 |
TSK-GEL | 0.01 | 0.9 | 90.0 | 1.2 | 300 |