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Table 2 Purification of alliinase from E. adhaerens FERM P-19486

From: Alliinase from Ensifer adhaerens and Its Use for Generation of Fungicidal Activity

Purification step

Protein

(mg)

Total activity

(units)

Sp. Act

(units/mg)

Yield

(%)

Purification

(fold)

Crude extract

209.81

73.0

0.3

100

1

DEAE-cellulose

9.60

30.0

3.1

41.1

10

Phenyl-sepharose

1.94

22.4

11.5

30.7

38

Aminohexyl-sepharose

0.29

7.5

25.9

10.3

86

Mono Q

0.04

4.1

102.5

5.6

341

TSK-GEL

0.01

0.9

90.0

1.2

300