AMB Express

Table 3 Organic solvent tolerance of B. subtilis GRSW2-B1 and B. subtilis 168

From: Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host

		Cell viability^a
Organic solvent ^b	Log P _ow ^c	*B. subtilis* 168	*B. subtilis* GRSW2-B1	*B. subtilis* GRSW2-B1 /pHZT-PX	*B. subtilis* GRSW2-B1 /pHZK-PX
None (control)	-	+++++++++	+++++++++	+++++++++	+++++++++
Octane	5.18	++++	+++++	++++	++++
Heptane	4.66	++++	+++++	++++	+++
Decanol	4.23	±	++++	++	++++
Hexane	3.90	+++	+++	++	+++
Nonanol	3.77	±	++++	±	+++
Cyclohexane	3.44	++	+++	++	+++
m-Xylene	3.20	++	++++	±	+++
p-Xylene	3.15	++	++++	±	+++
o-Xylene	3.12	++	++++	±	+++
Octanol	3.00	++	++++	±	+++
Toluene	2.73	++	++++	±	++++
Heptanol	2.62	±	++++	±	++++
Benzene	2.13	++	++++	±	+++
Hexanol	2.03	++	++++	±	++++
Butyl acetate	1.78	++	+++	±	+++
Pentanol	1.51	±	++++	±	++++
Butanol	0.88	±	++++	±	++++
Ethyl acetate	0.73	+++	++++	±	+++
THF (160 mM)	0.46	+++++++++	+++++++++	+++++++++	++++++++
Propanol	0.25	++++++	+++++	+++++++	+++++
2-Propanol	0.05	++++++++	++++++++	++++++++	++++++++
Ethanol	-0.31	+++++++++	+++++++++	+++++++++	++++++++
Acetonitrile	-0.34	++++++++	++++++++	++++++++	+++++++++
Methanol	-0.77	++++++++	+++++++++	++++++++	+++++++++
DMSO	-1.35	+++++++++	+++++++++	++++++++	++++++++

^aCells were initially grown to late-exponential phase in LB medium before organic solvent (5% v) was added. Cell viability was examined after 6 h of solvent exposure. The number of viable cells is represented by symbols +. The number of plus sign is corresponded to cell numbers (CFU.ml^-1): ± ( < 1 × 10²); ++ (1 - 9 × 10²); +++ (1 - 9 × 10³); ++++ (1 - 9 × 10⁴); +++++ (1 - 9 × 10⁵); ++++++ (1 - 9 × 10⁶); +++++++ (1 - 9 × 10⁷); ++++++++ (1 - 9 × 10⁸); +++++++++ (1 - 9 × 10⁹). Data are means of the results from at least three individual experiments.
^b THF, tetrahydrofuran; DMSO, dimethylsulfoxide.
^c Log P _ow value was obtained from KOW WIN version 1.67, EPI suite (U.S. Environmental Protection Agency).

Back to article page