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Table 3 Organic solvent tolerance of B. subtilis GRSW2-B1 and B. subtilis 168

From: Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host

   Cell viabilitya
Organic solvent b Log P ow c B. subtilis 168 B. subtilis GRSW2-B1 B. subtilis GRSW2-B1
/pHZT-PX
B. subtilis GRSW2-B1
/pHZK-PX
None (control) - +++++++++ +++++++++ +++++++++ +++++++++
Octane 5.18 ++++ +++++ ++++ ++++
Heptane 4.66 ++++ +++++ ++++ +++
Decanol 4.23 ± ++++ ++ ++++
Hexane 3.90 +++ +++ ++ +++
Nonanol 3.77 ± ++++ ± +++
Cyclohexane 3.44 ++ +++ ++ +++
m-Xylene 3.20 ++ ++++ ± +++
p-Xylene 3.15 ++ ++++ ± +++
o-Xylene 3.12 ++ ++++ ± +++
Octanol 3.00 ++ ++++ ± +++
Toluene 2.73 ++ ++++ ± ++++
Heptanol 2.62 ± ++++ ± ++++
Benzene 2.13 ++ ++++ ± +++
Hexanol 2.03 ++ ++++ ± ++++
Butyl acetate 1.78 ++ +++ ± +++
Pentanol 1.51 ± ++++ ± ++++
Butanol 0.88 ± ++++ ± ++++
Ethyl acetate 0.73 +++ ++++ ± +++
THF (160 mM) 0.46 +++++++++ +++++++++ +++++++++ ++++++++
Propanol 0.25 ++++++ +++++ +++++++ +++++
2-Propanol 0.05 ++++++++ ++++++++ ++++++++ ++++++++
Ethanol -0.31 +++++++++ +++++++++ +++++++++ ++++++++
Acetonitrile -0.34 ++++++++ ++++++++ ++++++++ +++++++++
Methanol -0.77 ++++++++ +++++++++ ++++++++ +++++++++
DMSO -1.35 +++++++++ +++++++++ ++++++++ ++++++++
  1. aCells were initially grown to late-exponential phase in LB medium before organic solvent (5% v) was added. Cell viability was examined after 6 h of solvent exposure. The number of viable cells is represented by symbols +. The number of plus sign is corresponded to cell numbers (CFU.ml-1): ± ( < 1 × 102); ++ (1 - 9 × 102); +++ (1 - 9 × 103); ++++ (1 - 9 × 104); +++++ (1 - 9 × 105); ++++++ (1 - 9 × 106); +++++++ (1 - 9 × 107); ++++++++ (1 - 9 × 108); +++++++++ (1 - 9 × 109). Data are means of the results from at least three individual experiments.
  2. b THF, tetrahydrofuran; DMSO, dimethylsulfoxide.
  3. c Log P ow value was obtained from KOW WIN version 1.67, EPI suite (U.S. Environmental Protection Agency).