Table 1 Bacterial strains and plasmids used in this study
From: Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host
Bacterial strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
B. subtilis GRSW2-B1 | Butanol-tolerant bacterium | This study |
B. subtilis 168 | A type-strain Bacillus subtilis. Source of promoter sequences: P43, P2L | Laboratory stock |
E. coli DH5α | hsdR17 recA endA1 lacZΔM15. For plasmid construction and propagation purpose | Invitrogen, USA |
Plasmids | ||
pHY300PLK | A shuttle vector for E. coli and B. subtilis, carrying bla (Apr) and tetL (Tcr). Source of tetracycline promoter (PTet) | (Ishiwa and Shibahara 1985) |
pQF50 | A broad-host range vector. Source of trpA terminators, a multiple cloning site (MCS) and lacZ | |
pUC4K | A vector carrying Apr, Kmr. Source of kanamycin promoter (PKm) | Laboratory stock |
pNCMO2 | A vector carrying strong promoter P2 for Brevibacillus. Source of P2 promoter (P2N) | Takara Bio Inc., Japan |
pDG148 | A shuttle vector for E. coli and B. subtilis, carrying bla (Apr) and kan (Kmr). Source of kanamycin resistant gene cassette and Spac promoter (PSpac) | Laboratory stock |
pWH1520 | An expression vector for B. megaterium. Source of xylose promoter (PxylA) | Mo Bi Tec, Germany |
pHZT | pHY300PLK (Tcr) carrying trpA, MCS, lacZ | This study |
pHZK | pHY300PLK, carrying trpA, MCS, lacZ, and tetL (Tcr) was replaced with kan (Kmr) | This study |
pHZT-P43 | pHZT carrying P43 | This study |
pHZT-PK | pHZT carrying PKm | This study |
pHZT-P2N | pHZT carrying P2N | This study |
pHZT-P2L | pHZT carrying P2L | This study |
pHZT-PT | pHZT carrying PTet | This study |
pHZT-PS | pHZT carrying PSpac | This study |
pHZT-PX | pHZT carrying PxylA | This study |
pHZK-PX | pHZK carrying PxylA | This study |