Figure 2

Promoter-driven β-galactosidase activity. B. subtilis GRSW2-B1, harboring each constructed expression vectors, was grown in LB medium to the same OD₆₀₀ of approximately 0.8, and induced with the optimal induction condition of each promoter (if necessary) (as described in text). pHZT and pHZTK is pHY300PLK, carrying trpA, MCS, lacZ, with Tc^r and Km^r, respectively. P43, PK, P2N, P2L, PT, PS, PX are P₄₃, P_kan, P_2N, P_2L, P_TetL, P_Sapc, and P_xylA promoters (as described in details in Table 2). BtOH is butanol, which was added at 1% v/v. Inset is the enlarged y-axis scale to elaborate differences of the first eight data values. Data are means of the results from at least three individual experiments.