Characterization of diverse natural variants of CYP102A1 found within a species of Bacillus megaterium

AMB Express

Table 1 Bacillus megaterium strains used in this study, and GenBank accession numbers for CYP102A1 variants, 16S rRNA, and ITS sequences between 16S-23S sequences^a

^aGenBank accession numbers (except J04832) were assigned to nucleotide sequences determined in this study. The corresponding CYP102A1 variant gene for each strain is listed.
^bThe CYP102A1 variants were named based on the amino acid similarity (Fig. 1a and Table 2).
^cPreviously known as the nucleotide sequence of P450 BM3 (CYP102A1) from B. megaterium (Ruettinger et al. 1989).
^dGenetic Information regarding the CYP102A1 variant of B. megaterium QM B1551 (ATCC 12872) was obtained from the Whole Genome Sequencing of B. megaterium http://www.bios.niu.edu/b_megaterium/ and the variant was designated as QM B1551. We only used its genetic information to compare to those of other variants and did not study its biochemical and physical properties.
^eGenetic information of B. megaterium QM B1551 (ATCC 12872) regarding its CYP102A1 variant, 16S rRNA, and ITS was obtained from the Whole Genome Sequencing of B. megaterium http://www.bios.niu.edu/b_megaterium/. Accession numbers were not provided.