Microbial community analysis in biocathode microbial fuel cells packed with different materials
© Sun et al; licensee Springer. 2012
Received: 23 February 2012
Accepted: 29 March 2012
Published: 29 March 2012
Biocathode MFCs using microorganisms as catalysts have important advantages in lowering cost and improving sustainability. Electrode materials and microbial synergy determines biocathode MFCs performance. In this study, four materials, granular activated carbon (GAC), granular semicoke (GS), granular graphite (GG) and carbon felt cube (CFC) were used as packed cathodic materials. The microbial composition on each material and its correlation with the electricity generation performance of MFCs were investigated. Results showed that different biocathode materials had an important effect on the type of microbial species in biocathode MFCs. The microbes belonging to Bacteroidetes and Proteobacteria were the dominant phyla in the four materials packed biocathode MFCs. Comamonas of Betaproteobacteria might play significant roles in electron transfer process of GAC, GS and CFC packed biocathode MFCs, while in GG packed MFC Acidovorax may be correlated with power generation. The biocathode materials also had influence on the microbial diversity and evenness, but the differences in them were not positively related to the power production.
KeywordsBiocathode microbial fuel cell Cathodic materials Electricity generation Microbial community
Microbial fuel cells (MFCs) utilize microorganisms as catalysts, which can promote biodegradation of organic matters and simultaneously produce an electrical current (Bond et al. 2002). In the past few years, researchers generally use chemical cathode MFC to remove the organic carbon in wastewater, but the cost of chemical cathode is high and it is easily lead to pollution. Currently, biocathode MFCs using microorganisms instead of common Pt as catalysts have important advantages in lowering cost, expanding function and improving sustainability. Therefore, biocathode MFCs as a new economical and environmentally friendly wastewater treatment technology has drawn more and more attentions (Huang et al. 2011). Although biocathode MFCs have many advantages, the current studies are still at laboratory level. The main challenge for their large-scale application is low power generation capability. Microorganisms are the core of biocathode MFCs. In the anode, microorganisms attaching on the electrode material and forming biofilm play an essential role in MFC generating electricity (Rabaey and Rozendal 2010), and in the cathode, the microbial catalytic efficiency plays a key role to improve the cathode potential and power output (Osman et al. 2010). Therefore, better understanding of the ecology of the microbial communities in the different reactors will be helpful to improve MFCs power production.
At present, the anodic microbes get more attention, including the electricity-producing bacteria species (Holmes et al. 2004Xia et al. 2010), anodic microbial community composition (Cárcer et al. 2011Jung and Regan 2010Kim et al. 2011Zhang et al. 2011), the mechanism of extracellular electron transfer (Carmona-Martinez et al. 2011Strycharz et al. 2011) and so on. In contrast, the researches on the microbes of biocathode MFCs are very limited, and mainly focused on the role of pure bacteria in biocathode MFCs. For instance, Carbajosa et al. (2010) found that an acidophilic Acidithiobacillus ferrooxidans could promote oxygen reduction in biocathode MFCs. Mao et al. (2010) reported that the power generation from a biocathode MFC was biocatalyzed by ferro/manganese-oxidizing bacteria. Recently, a research analyzed the microbial community and electron transfer, when nitrate was used as electron acceptor (Chen et al. 2010). However, electrode materials and microbial synergy determines biocathode MFCs performance. Different electrode materials have certain differences in conductivity, surface area and porosity. These differences may affect the cathode microbial adhesion and growth. However, the influence of different biocathode materials on the microbial composition is still unknown.
In our previous study (Wei et al. 2011), two types of relative cheaper electrode materials, granular semicoke (GS) and granular activated carbon (GAC), as biocathode packed materials, and the material characteristic, electrochemical performance and price-performance ratio were compared with carbon felt cube (CFC) and granular graphite (GG). Results indicated that MFCs with GS and GAC outperformed MFCs with GG and CFC biocathode. But the dominate microorganisms in different biocathode materials were not analyzed and the interaction mechanism between microbes and biocathode materials was unclear.
The objective of this study is to analyze the microbial community composition attaching on the four biocathode materials, illustrate the predominate microbes on each biocathode materials and analyze the relationship between microorganisms and power generation in biocathode MFCs.
Materials and methods
MFC construction and operation
Four double-chambered flat plate MFCs with same size were built. Each MFC had two compartments with a total volume of 100 mL (2 cm thickness, 50 cm2 cross section), which were separated by an Ultrex cation exchange membrane (CMI-7000, Membranes International, USA). The titanium mesh was placed next to the cation exchange membrane, which was used to gather electrons flowing in each chamber. The titanium sheet was served as a lead to connect both electrodes and external resistance. Four biocathode materials (CFC, GG, GAC and GS) were filled in separate cathodic compartments, and anodic compartments of all four MFCs were filled with the same CFC used in cathode. The anodic and cathodic compartments were inoculated with microbial consortiums previously enriched in biocathode MFCs that had been operated in fed-batch mode for over three months. Two saturated calomel electrodes (SCE, 0.242 V vs. standard hydrogen electrode (SHE), Leici, China) were fitted through the rubber stopper of anodic and cathodic compartments respectively to be used as reference electrodes. The medium in the anodic compartment consisted of 1.64 g/L CH3COONa, 1.5 g/L NH4Cl, 3.4 g/L KH2PO4, 4.4 g/L KH2PO4, 0.1 g/L CaCl2·2H2O and 0.1 g/L MgCl2·6H2O. The cathode was fed with a similar medium with the anode, except that 1.64 g/L CH3COONa was replaced with 1.90 g/L NaHCO3. The nutrient solution was pumped to anodic and cathodic compartments separately at a rate of 20 mL/min using a peristaltic pumps (BT100-1 L, Baoding Longer Precision Pump Co., Ltd., China). To provide abundant oxygen for the cathodic reaction, air was continuously sparged into a conical flask which was connected with the vessel of cathode recirculation. MFCs were operated in fed-batch mode, and the anolyte and catholyte were replaced every 3 days. All reactors were operated at ambient temperature (28 ± 3°C) with a 1000 Ω resistor connected unless otherwise specified.
Material characteristics and electrochemical analysis
The specific surface area of four cathodic materials was measured with a micropore surface area and pore size analyzer (Autosorb-1 MP, Quantachrome Instrumants, USA).
The voltage across an external resistor was measured at a time interval of 20 min using a data acquisition system (DAQ2213, ADLINK, Beijing, China). The power densities were calculated from P = IE/V, where I is the current (=E/R), E the measured voltage, R is the external resistance, P the power density, and V the net liquid volume of the anodic compartment (Logan et al., 2006).
Bacterial community analysis
Biofilms attaching on the biocathode materials in MFCs were sampled at the end of the experiment, and rinsed with sterile distilled water to remove loosely attached bacteria. The cathodic biofilm DNA was extracted using a PowerSoil DNA isolation kit (MO BIO Laboratories) and then the bacteria 16S rRNA was amplified. Bacterial universal primers 27f (5'-AGA GTT TGA TCM TGG CTC AG-3') and 1495r (5'-CTA CGG CTA CCT TGT TAC GA-3') (Barberio et al. 2001) were used, and the PCR conditions were carried out in accordance with the reference (Di Cello et al. 1997). The products of triplicate PCR amplifications from each sample were joined and purified with Wizard® SV Gel and PCR Clean-Up System (Promega). The purified product was ligated into the pGEM®-T Easy vector (Promega) and the resulting plasmids were used to transform competent E. coli JM109 cells. Transformants were screened using blue-white selection on Luria agar containing
X-gal/IPTG and 100 mg/mL ampicillin. White colonies were selected, transferred to fresh ampicillin-supplemented plates and incubated overnight at 37°C. Plasmids were extracted using EZ Spin Column Plasmid Mini-Preps Kit (Shanghai Sangon). Positive clones were sequenced with primers T7/SP6 by a DNA sequencer (ABI 3730). Clone sequences were identified by comparison to the Genbank nucleotide database using BLAST via the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov/BLAST/). Mega 4.0.2 (Tamura et al. 2007) was used to align these sequences, and the neighbor-joining method was employed to generate a phylogenetic tree with a bootstrap test (1000 replicates) of phylogeny. Sequences obtained in this study have been deposited in GenBank with accession numbers of JN541127-JN541192 and JN565978. Sequences with greater than 97% identity are typically assigned to the same species (Schloss and Handelsman 2005). Rarefaction curves were used to determine if a sufficient number of clones from each of the libraries had been sequenced (http://strata.uga.edu/software/Software.html). The sampling coverage value, diversity (Shannon-Wiener index) and evenness were calculated as previously described (Gremion et al. 2003).
The maximum power densities of four different cathodic materials packed MFCs are shown in Additional file 1: Table S1. Results indicated that as cathodic material, the power density of GAC was the highest (24.3 W/m3), followed by GS (20.1 W/m3), CFC (17.1 W/m3), and GG (14.4 W/m3).
Diversity estimation and clone library comparison
Bacterial 16S rRNA clone libraries from different cathode material packed in MFCs
Positive clones number
Shannon index (H')
Microbial community distribution
The maximum power densities of GAC, GS,CFC and GG packed MFCs showed a decreasing trend. The specific areas changed in the similar way as the power densities of MFCs with different cathodic materials. It indicated that the power density had positive correlation with specific area, which can be assured that high specific area is profitable for microbial attachment and biological catalytic processes.
Shannon diversity indices results suggested that the diversity was the highest on GS and lowest on GAC. The results showed that biocathode materials had effects on the microbial diversity and evenness. Results on electrical power showed that the power density was the highest in GAC packed MFC, followed by GS, CFC and GG (Additional file 1: Table S1). It indicated that the microbial diversity and evenness in biocathode material was not positively related to the power production. Other studies about anodic microbes also showed that numerical abundance of microorganisms in anodic biofilms was not correlated with the current (Kiely et al. 2010).
Phylogenetic analysis indicated that the biofilm developing on each cathodic surface had some differences. It could be assumed that the microbial members had different affinities for the materials. Bacteroidetes and Betaproteobacteria were predominant in the biofilms sampled from four cathode materials. In a related study, it was also reported that the microbial community composition of an oxygen reducing biocathode was dominated by Bacteroidetes (Rabaey et al. 2008). When nitrate was served as the terminal electron acceptor on the biocathode, Chen et al. (2010) analyzed the microbial community dynamics and the results showed that Betaproteobacteria and Bacteroidetes were the most abundant division of the community. Another study showed that Gammaproteobacteria was the most abundant, followed by uncultured Bacteria and Bacteroidetes (Chen et al. 2008). These results indicated that the microorganisms belonging to proteobacteria and Bacteroidetes play important roles in catalyzing oxygen or nitrate reduction in cathodic compartment.
Electrode materials had an important effect on the type of microbial species in MFCs reactors. Different electrode materials have different microscopic surface structure and conductivity, which in turn affects the adhesion of specific microbes (Rabaey et al. 2004Zhou et al. 2011). In GAC, GS and CFC packed MFCs, Comamonas of Betaproteobacteria was the dominated genus; Acidovorax was the most obvious microbes of GG packed MFC. The powder density was the highest in GAC packed MFC, followed by GS, CFC and GG. Therefore microbes Comamonas and its abundance in cathodic materials might have some positive relation to the power generation in GAC, GS and GG packed MFCs, while in GG packed MFC Acidovorax may be correlated with electron transfer. A recent study showed that Comamonas testosteroni displayed a higher power production performance under a high pH condition in the anode chamber (Juang et al. 2011). The similarities between anode and cathode reducing/oxdizing populations may indicate the capability of many organisms to perform electron transfer both to and from electrodes, such as Shewanella putrefaciens and Geobacter sulfurreducens. Some electrochemically active bacteria in biocathodes have been reported, including Gram-negative and positive bacteria, such as Acinetobacter calcoaceticus, Sphingobacterium multivorum, Micrococcus luteus and Bacillus subtilis, which can catalyze oxygen reduction in biocathode MFCs (Rabaey et al. 2008Cournet et al. 2010). Therefore, the cathodic biofilm in a MFC is composed of diverse populations of bacteria, and they may work together to electron transfer and power production.
In conclusion, electrical results showed that the power density was the highest in GAC packed MFC, followed by GS, CFC and GG. Different biocathode materials had effects on the microbial diversity and evenness, but the differences in microbial diversity and evenness of different biocathode materials were not positively related to the power production. Biocathode materials had an important effect on the type of microbial species in MFCs reactors. The microbes belonging to Bacteroidetes and Proteobacteria were the dominant phyla in the four materials packed biocathode MFCs. Comamonas of Betaproteobacteria might have important effects on electron transfer process of GAC, GS and CFC packed biocathode MFCs, while in GG packed MFC Acidovorax may be correlated with power generation.
This research was supported by National High Technology Research and Development Program of China (863 Program) (No. 2009AA06Z306) and China Postdoctoral Science Foundation (023208015).
- Barberio C, Pagliai L, Cavalieri D, Fani R: Biodiversity and horizontal gene transfer in culturable bacteria isolated from activated sludge enriched in nonylphenol ethoxylates. Res Microbiol 2001,152(1):105–112. 10.1016/S0923-2508(00)01173-6View ArticlePubMedGoogle Scholar
- Bond DR, Holmes DE, Tender LM, Lovley DR: Electrode-reducing microorganisms that harvest energy from marine sediments. Science 2002,295(5554):483–485. 10.1126/science.1066771View ArticlePubMedGoogle Scholar
- Cárcer DA, Ha PT, Jang JK, Chang IS: Microbial community differences between propionate-fed microbial fuel cell systems under open and closed circuit conditions. Appl Microbiol Biotechnol 2011,89(3):605–612. 10.1007/s00253-010-2903-xView ArticlePubMedGoogle Scholar
- Carmona-Martinez AA, Harnisch F, Fitzgerald LA, Biffinger JC, Ringeisen BR, Schröder U: Cyclic voltammetric analysis of the electron transfer of Shewanella oneidensis MR-1 and nanofilament and cytochrome knock-out mutants. Bioelectrochem 2011,81(2):74–80. 10.1016/j.bioelechem.2011.02.006View ArticleGoogle Scholar
- Carbajosa S, Malki M, Caillard R, Lopez MF, Palomares FJ, Martín-Gago JA, Rodríguez N, Amils R, Fernández VM, Lacey ALD: Electrochemical growth of Acidithiobacillus ferrooxidans on a graphite electrode. Biosens Bioelectron 2010,26(2):887–880.View ArticleGoogle Scholar
- Chen GW, Choi SJ, Lee TH, Lee GY, Cha JH, Kim CW: Application of biocathode in microbial fuel cells: cell performance and microbial community. Appl Microbiol Biotechnol 2008,79(3):379–388. 10.1007/s00253-008-1451-0View ArticlePubMedGoogle Scholar
- Chen GW, Choi SJ, Lee TH, Lee GY, Cha JH, Kim CW: Microbial community dynamics and electron transfer of a biocathode in microbial fuel cells. Korean J Chem Eng 2010,27(5):1513–1520. 10.1007/s11814-010-0231-6View ArticleGoogle Scholar
- Cournet A, Délia M-L, Bergel A, Roques C, Bergé M: Electrochemical reduction of oxygen catalyzed by a wide range of bacteria including Gram -positive. Electrochem Commun 2010,12(4):505–508. 10.1016/j.elecom.2010.01.026View ArticleGoogle Scholar
- Di Cello F, Bevivino A, Chiarini L, Fani R, Paffetti D, Tabacchioni S, Dalmastri C: Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages. Appl Environ Microbiol 1997,63(11):4485–4493.PubMed CentralPubMedGoogle Scholar
- Gremion F, Chatzinotas A, Harms H: Comparative 16S rDNA and 16S rRNA sequence analysis indicates that Actinobacteria might be a dominant part of the metabolically active bacteria in heavy metalcontaminated bulk and rhizosphere soil. Environ Microbiol 2003,5(10):896–907. 10.1046/j.1462-2920.2003.00484.xView ArticlePubMedGoogle Scholar
- Holmes DE, Nicoll JS, Bond DR, Lovley DR: Potential Role of a Novel Psychrotolerant Member of the Family Geobacteraceae, Geopsychrobacter electrodiphilus gen. nov., sp. nov., in Electricity Production by a Marine Sediment Fuel Cell. Appl Environ Microbiol 2004,70(10):6023–6030. 10.1128/AEM.70.10.6023-6030.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Huang L, Regan JM, Quan X: Electron transfer mechanisms, new applications, and performance of biocathode microbial fuel cells. Bioresour Technol 2011,102(1):316–323. 10.1016/j.biortech.2010.06.096View ArticlePubMedGoogle Scholar
- Jung S, Regan JM: Influence of External Resistance on Electrogenesis, Methanogenesis, and Anode Prokaryotic Communities in Microbial Fuel Cells. Appl Environ Microbiol 2010,77(2):564–571.PubMed CentralView ArticlePubMedGoogle Scholar
- Juang DF, Yang PC, Chou HY, Chiu LJ: Effects of microbial species, organic loading and substrate degradation rate on the power generation capability of microbial fuel cells. Biotechnol Lett 2011,33(11):2147–2160. 10.1007/s10529-011-0690-9View ArticlePubMedGoogle Scholar
- Kiely PD, Call DF, Yates MD, Regan JM, Logan BE: Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera. Appl Microbiol Biotechnol 2010,88(1):371–380. 10.1007/s00253-010-2757-2View ArticlePubMedGoogle Scholar
- Kim JR, Beecroft NJ, Varcoe JR, Dinsdale RM, Guwy AJ, Slade RCT, Thumser A, Avignone-Rossa C, Premier GC: Spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell. Appl Microbiol Biotechnol 2011,90(3):1179–1191. 10.1007/s00253-011-3181-yView ArticlePubMedGoogle Scholar
- Logan BE, Hamelers B, Rozendal R, Schroder U, Keller J, Freguia S, Aelterman P, Verstraete W, Rabaey K: Microbial fuel cells: methodology and technology. Environ SciTechnol 2006,40(17):5181–5192. 10.1021/es0605016View ArticleGoogle Scholar
- Mao Yp, Zhang LH, Li DM, Shi HF, Liu YD, Cai LK: Power generation from a biocathode microbial fuel cell biocatalyzed by ferro/manganese-oxidizing bacteria. Electrochim Acta 2010,55(27):7804–7808. 10.1016/j.electacta.2010.03.004View ArticleGoogle Scholar
- Osman MH, Shah AA, Walsh FC: Recent progress and continuing challenges in bio-fuel cells. Part II: Microbial. Biosens Bioelectron 2010,26(3):953–963. 10.1016/j.bios.2010.08.057View ArticlePubMedGoogle Scholar
- Rabaey K, Boon N, Siciliano SD, Verhaege M, Verstraete W: Biofuel cells select for microbial consortia that self-mediate electron transfer. Appl Environ Microbiol 2004,70(9):5373–5382. 10.1128/AEM.70.9.5373-5382.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Rabaey K, Read ST, Clauwaert P, Freguia S, Bond PL, Blackall LL, Keller J: Cathodic oxygen reduction catalyzed by bacteria in microbial fuel cells. ISME J 2008,2(5):519–527. 10.1038/ismej.2008.1View ArticlePubMedGoogle Scholar
- Rabaey K, Rozendal RA: Microbial electrosynthesis-revisiting the electrical route for microbial production. Nat Rev Micro 2010,8(10):706–716. 10.1038/nrmicro2422View ArticleGoogle Scholar
- Schloss PD, Handelsman J: Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Appl Environ Microbiol 2005,71(3):1501–1506. 10.1128/AEM.71.3.1501-1506.2005PubMed CentralView ArticlePubMedGoogle Scholar
- Strycharz SM, Glaven RH, Coppi MV, Gannon SM, Perpetua LA, Liu A, Nevin KP, Lovley DR: Gene expression and deletion analysis of mechanisms for electron transfer from electrodes to Geobacter sulfurreducens . Bioelectrochem 2011,80(2):142–150. 10.1016/j.bioelechem.2010.07.005View ArticleGoogle Scholar
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Bio Evol 2007,24(8):1596–1599. 10.1093/molbev/msm092View ArticleGoogle Scholar
- Wei JC, Liang P, Cao XX, Huang X: Use of inexpensive semicoke and activated carbon as biocathode in microbial fuel cells. Bioresour Technol 2011,102(22):10431–10435. 10.1016/j.biortech.2011.08.088View ArticlePubMedGoogle Scholar
- Xia X, Cao XX, Liang P, Huang X, Yang SP, Zhao GG: Electricity generation from glucose by a Klebsiella sp. in microbial fuel cells. Appl Microbiol Biotechnol 2010,87(1):383–390. 10.1007/s00253-010-2604-5View ArticlePubMedGoogle Scholar
- Zhang Y, Min B, Lp H, Angelidaki I: Electricity generation and microbial community response to substrate changes in microbial fuel cell. Bioresour Technol 2011,102(2):1166–1173. 10.1016/j.biortech.2010.09.044View ArticlePubMedGoogle Scholar
- Zhou M, Chi M, Luo J, He H, Jin T: An overview of electrode materials in microbial fuel cells. J Power Sources 2011,196(10):4427–4435. 10.1016/j.jpowsour.2011.01.012View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.