Fermentation, Isolation, Structure, and antidiabetic activity of NFAT-133 produced by Streptomyces strain PM0324667
- Asha A Kulkarni-Almeida1Email author,
- Manoja K Brahma1,
- Prabhu Padmanabhan1,
- Prabhu D Mishra1,
- Rajashri R Parab1,
- Nitin V Gaikwad1,
- Chandni S Thakkar1,
- Pradipta Tokdar1,
- Prafull V Ranadive1,
- Amrutha S Nair1,
- Anagha A Damre1,
- Umakant A Bahirat1,
- Nitin J Deshmukh1,
- Lalit S Doshi1,
- Amol V Dixit1,
- Saji D George1,
- Ram A Vishwakarma2,
- Kumar VS Nemmani1 and
- Girish B Mahajan1Email author
© Kulkarni-Almeida et al; licensee Springer. 2011
Received: 4 November 2011
Accepted: 21 November 2011
Published: 21 November 2011
Type-2 diabetes is mediated by defects in either insulin secretion or insulin action. In an effort to identify extracts that may stimulate glucose uptake, similar to insulin, a high throughput-screening assay for measuring glucose uptake in skeletal muscle cells was established. During the screening studies to discover novel antidiabetic compounds from microbial resources a Streptomyces strain PM0324667 (MTCC 5543, the Strain accession number at Institute of Microbial Technology, Chandigarh, India), an isolate from arid soil was identified which expressed a secondary metabolite that induced glucose uptake in L6 skeletal muscle cells. By employing bioactivity guided fractionation techniques, a tri-substituted simple aromatic compound with anti-diabetic potential was isolated. It was characterized based on MS and 2D NMR spectral data and identified as NFAT-133 which is a known immunosuppressive agent that inhibits NFAT-dependent transcription in vitro. Our investigations revealed the antidiabetic potential of NFAT-133. The compound induced glucose uptake in differentiated L6 myotubes with an EC50 of 6.3 ± 1.8 μM without activating the peroxisome proliferator-activated receptor-γ. Further, NFAT-133 was also efficacious in vivo in diabetic animals and reduced systemic glucose levels. Thus it is a potential lead compound which can be considered for development as a therapeutic for the treatment of type-2 diabetes. We have reported herewith the isolation of the producer microbe, fermentation, purification, in vitro, and in vivo antidiabetic activity of the compound.
KeywordsNFAT-133 Streptomyces sp. Antidiabetic Actinomycetes
In 2010, the global prevalence of diabetes was estimated to have reached 285 million and it is predicted to reach 438 million in 2030. Available agents provide imperfect control of the disease, and the medical need for better therapies is widely recognized (Norman 2010). About 90% to 95% of patients have non-insulin dependent diabetes mellitus (NIDDM) or type-2 diabetes and the standard therapy for the treatment of NIDDM has its own limitations (Mark 1997). Diet, oral hypoglycaemic drugs and insulin are the standard modes of treatment. However they are unable to achieve relief from diabetes. This leads to acute and chronic complications. Hence major efforts have been directed towards development of oral hypoglycaemic drugs, to identify both novel insulin secretagogues and compounds able to enhance insulin action in target tissues.
High throughput screening of natural product libraries had previously been attempted by us as an approach towards identifying novel scaffolds with potent biological activity (Kulkarni-Almeida et al. 2008). Several pharmaceutical industries use this approach to investigate natural product based library collections (Quinn et al. 2002). A major segment of these biota collections were represented by microbial cultures. Among the microbial resources actinomycetes have been proven to be a rewarding source of antidiabetic compounds. Acarbose, voglibose, valienamine, adiposin-1, and trestatin-B were reported from Actinoplanes utahensis (Schmidit et al. 1977), Streptomyces hygroscopicus-limoneus (De Melo et al. 2006; Kameda et al. 1980), S. calvus (Mahmud 2003; Truscheit et al. 1981), and S. dimorphogenes (Yokose et al. 1983; Yokose et al. 1984) respectively. Acarbose is an oral alpha-glucosidase and alpha-amylase inhibitor that was first launched by Bayer in Switzerland in 1989 for the oral treatment of type-2 diabetes mellitus (Schmidit et al. 1977). Voglibose is an alpha-glucosidase inhibitor used for lowering post-prandial blood glucose levels in people with diabetes mellitus. It is produced and marketed in India by trade name Volix® and Vocarb®(De Melo et al. 2006). Valielamine, a precursor of voglibose and a new aminocyclitol were isolated from the fermentation broth of Streptomyces hygroscopicus subspecies limoneus. It has more potent α-glucosidase inhibitory activity against porcine intestinal sucrase, maltase and isomaltase than valienamine, validamine and hydroxyl-validamine which were reported as building blocks of validamycins and microbial oligosaccharide α-glucosidase inhibitors (Kameda et al. 1984; Xu et al. 2009).
One of the hallmarks of type-2 diabetes is decreased sensitivity of cells to insulin. Our approach was to explore microbial collections for extracts with anti-diabetic metabolites that stimulate glucose uptake in skeletal muscle cells. Using this strategy we have previously identified plant extracts Aegle marmelos and Syzygium cumini which stimulate glucose uptake activity in L6 myotubes. This activity was mediated by activation of glucose uptake by glucose transporter 4 (GLUT4), peroxisome proliferator activator receptor gamma (PPAR-γ) and phosphatidylinositol 3' kinase (PI3K), (Anandharajan et al. 2006). Similarly, (Jung et al. (2006)) showed that Ganoderma lucidum extract stimulates glucose uptake activity in skeletal muscles by activating the regulatory molecules PI3-kinase and AMP activated protein kinase (AMPK). We explored the microbial metabolite extract library in search of compounds that enhance glucose uptake in skeletal muscle cells in presence of insulin. Upon insulin treatment of skeletal muscles, the insulin receptor is phosphorylated which activates a signal transduction pathway leading to increased translocation and GLUT4 (Yonemitsu et al. 2001). These features of L6 myotubes are critical since GLUT4 is responsible for insulin-dependent glucose uptake in the mature differentiated skeletal muscle cell.
High throughput screening of diverse microbial metabolite extract library was conducted for detecting glucose uptake inducers in rat skeletal muscle cells. The extract library is prepared from diverse microbes isolated from variety of ecological units in India. 880 extracts representing Actinomycetes strains yielded eight actives. These could stimulate glucose uptake in differentiated skeletal muscle cells. The extract of an actinomycetes strain PM0324667 of the genera Streptomyces showed reproducible glucose uptake activity through several refermentation generations. Using fermented broth of this strain, bioactivity guided fractionation was conducted to isolate the active principle. We isolated the compound NFAT-133, which was earlier, reported as an immunosuppressive agent. This compound induced significant glucose uptake in rat skeletal muscle in presence of insulin. The activity was possibly mediated through a PPAR-γ independent mechanism. Further, the compound showed a significant reduction in plasma glucose and plasma insulin levels in diabetic (db/db) mice as compared to vehicle treated animals. In this paper, we report the antidiabetic activity of NFAT-133 along with isolation of its producer microorganism, identification, fermentation, and structure elucidation.
Materials and methods
Isolation of Streptomyces strain PM0324667
The Streptomyces strain PM0324667 was isolated from a sandy soil sample collected from Jaipur in Rajasthan, India. The soil was suspended in demineralised water (10% w/v) and heated at 55°C for 6 minutes. 100 μl of the suspension was spread on CSPYME agar consisting of casein (0.1%), corn starch (1.0%), K2HPO4, (0.05%), peptone (0.1%), yeast extract (0.1%), malt extract (1.0%), agar agar (1.5%), hydroxylamine (0.01%) and amphotericin-B (0.002%) with initial pH value of 7.0. The medium plates were incubated at 30°C for seven days. The colonies were transferred onto agarified ISP2 medium. ISP2 medium consisted of malt extract (1.0%), glucose (0.4%), yeast extract (0.4%) and agar agar (1.6%) with initial pH value of 7.0. The medium slants were incubated at 30°C for 7 days. Typical colonies of streptomycetes, with rough surface and chalky white or gray in color were picked-up on ISP2 agar plates. 47 colonies were isolated and purified from the arid soil sample. All 47 cultures from arid soils were screened in the in vitro antidiabetic assay. Streptomyces strain PM0324667 was one these 47 which showed consistent and reproducible antidiabetic activity.
Microscopic and macroscopic features of the strain PM0324667 were examined by the classical microbiological techniques. Total DNA sample of the strain was prepared as reported (Arun et al. 2005). The 16S ribosomal RNA gene (16S rRNA) was amplified by polymerase chain reaction (PCR) using genomic DNA and universal primers 243F (5'-GGA TGA GCC CGC GGC CTA-3') - forward primer and 1385R (5'-CGG TGT GTA CAA GGC CC-3') - reverse primer and sequenced (Arun et al. 2005). A homology search for the most nearest sequences was performed using the BLAST algorithm on the GenBank/EMBL/DDBJ/PDB.
Production of NFAT-133 by Streptomyces strain PM0324667
Streptomyces strain PM0324667 (MTCC 5543) from the ISP2 medium slant was inoculated into seed medium; 274-(1) consisting of glucose (1.5%), corn steep liquor (0.5%), peptone (0.75%), yeast extract (0.75%), calcium carbonate (0.2%) and sodium chloride (0.5%) with initial pH value of 7.0. The culture was incubated on a rotary shaker [240 revolutions per minute (rpm)] at 30°C (± 1°C) for three days. 5 ml of this seed was inoculated into 100 ml fermentation medium; 5189M consisting of malt extract (2.0%), yeast extract (0.2%), glucose (1.0%) and (NH4)2HPO4, (0.005%) with initial pH value of 6.0. The fermentation was carried out on a rotary shaker (240 rpm) at 30°C (± 1°C) for three days.
Extraction and isolation
The fermented broth was extracted with methanol (1:1, v/v) and it was stirred for 60 minutes and filtered through a coarse filter paper. The filtrate was concentrated using vacuum concentrator. The aqueous concentrate was passed through 10% (v/v) bed volume of Diaion® HP20 (SUPELCO) resin at the rate of 1 L/h. The adsorbent on the resin was eluted with 80% methanol after water wash. The methanolic extract was dissolved in demineralized water to get concentration of 75 mg/ml. 100 ml of this aqueous solution was partitioned with 100 ml ethyl acetate (EA) five times. The EA extracts were pooled together and dried on vacuum concentrator.
The EA concentrate was dry charged to CombiFlash chromatography instrument with pre packed Redisep silica gel column (10 g). The column was sequentially eluted with chloroform and methanol mixture (5-20% methanol) and the fractions monitored by thin layer chromatography (TLC). Similar fractions were pooled to get three samples namely; Fraction-1, Fraction-2 and Fraction-3. These samples along with crude extract of EA were screened for glucose uptake activity. The active Fraction-1 was subjected to reverse phase silica (C-18) cartridge, strata™ from Phenomenex and eluted with water: acetonitrile mixture in gradient mode. The pure compound was recovered from the 20% acetonitrile eluate, by evaporating acetonitrile and water using a high vacuum concentrator.
HPLC was performed using a Lichrosphere RP-18 column (125 × 4 mm) in a Shimadzu LC-2010CHT Liquid Chromatograph. The final purification was done on Waters PrepLC 4000 System. Solvents used for reverse-phase were of HPLC grade and normal-phase column chromatography was performed with demineralized commercial-grade solvents. Silica gel (SiO2; 200-300 mesh) was used for CC and GF254 (30-40 mm) TLC plates were procured from Merck. An NMR spectrum of the compound was recorded in CDCl3 on Bruker 300 MHz spectrometer with TMS as the internal standard. Chemical shift δ values were expressed in ppm, and coupling constant J in Hz. ESI LC-MS was recorded on Bruker Daltonics. CombiFlash® Sq 16× Teledyne Technologies Company ISCO attached with UV/VIS detector was used for Flash chromatography, using RediSep® Flash Column silica 12 g Teledyne ISCO.
Large scale fermentation
A well-sporulated seven days old slant culture was inoculated into 200 ml of seed medium consisting of glucose (1.5%), corn steep liquor (0.5%), peptone (0.75%), yeast extract (0.75%), calcium carbonate (0.2%), and sodium chloride (0.5%) in demineralized water with an initial pH value of 6.5-7.0 in 1 L erlenmeyer flasks. All the flasks were incubated on a rotary shaker for 3 days at 230-250 rpm at 30°C (± 1°C). 2.5 L seed was transferred to 150 L fermentor (B. Braun Melsungen AG) containing 100 L production medium consisting of malt extract (2.0%), yeast extract (2.0%), glucose (1.0%), di-ammonium hydrogen phosphate (0.005%) in demineralized water with initial pH value of 6.0 and fermented with the following parameters: aeration 0.5 vvm, temperature 30°C(± 1°C), agitation 100 rpm. 20% dextrose solution was fed at 24 h of the fermentation cycle at 5% (v/v) and the batch was harvested at 48 h. The whole broth was extracted with equal volume of ethyl acetate (EA). The EA extract was used for isolation of the compound NFAT-133.
Determination of in vitro activity
Cell lines and Reagents
The cell lines, media and reagents used in the assay are namely; alpha-MEM (Bioconcept), fetal bovine serum (Gibco), penicillin-streptomycin solution (Sigma),14C-2-deoxyglucose (GE Healthcare, UK)), 2-deoxyglucose (Sigma Aldrich, St. Louis, MO, USA), KRPH buffer (pH.7.4), Microscint PS (Perkin Elmer, USA), 96-well plates (Nunc), L6 rat skeletal muscle cells (CRL1458, ATCC, USA), human recombinant insulin (Sigma, St. Louis, MO, USA), rosiglitazone (synthesized in house).
The metabolite extract library samples were screened for the ability to induce glucose uptake in insulin stimulated differentiated L6 myotubes. The L6 rat skeletal muscle cells were seeded into 96 well cell culture plates in complete medium (α-MEM + 10% fetal bovine serum + 1% penicillin-streptomycin solution) and maintained at 5% carbon dioxide (CO2) atmosphere. After 48 h, the medium was switched to α-MEM with 2% (v/v) fetal bovine serum (FBS) in order to initiate differentiation. Media change was ensured every 48 h. Compound treatment was conducted once the myoblasts differentiated to form myotubes. The differentiated and fused myotubes were starved in serum free α-MEM for 4 h, treated with samples and further incubated for 24 h. Following treatment and incubation, the cells were sensitized with insulin (200 nM) and incubated for 25 minutes. Radioactive glucose pulsing was done at 0.06 μCi/ml for 10 minutes. The reaction was terminated with cold KRPH buffer. The cell-associated radioactivity was determined by lysing the cells with Microscint PS™ followed by liquid scintillation counting (Huang et al. 2002). The assay was performed in triplicate.
For all experiments, the L6 myoblasts obtained from ATCC were used only up to passage seven and were utilized within two months post revival of a new vial.
PPAR-γ luciferase assay
PPAR-γ activation is one of the key mechanisms involved in glucose uptake. To determine if NFAT-133 was exerting its effects by activation of PPAR-γ, a luciferase assay was performed using the expression vector for human PPAR-γ and the PPRE-tk-LUC reporter plasmid. CV1 cells were seeded onto 24 well plates and transient transfections were conducted after over-night incubation using lipofectamine (Invitrogen, CA, USA). Five hours after transfection, the cells were treated with each compound concentration for an additional 24 h, and the luciferase assay (Promega) was performed according to the manufacture's protocol. Luminescence was measured using the Safire2 reader. Transactivation of human PPAR-γ by 1 μM rosiglitazone is considered as 100% activation and hence data for NFAT-133 is calculated in comparison to rosiglitazone which was used as a positive control in the assay. The entire assay was performed in triplicates.
Determination of in vivo activity
Male db/db mice (7-9 weeks of age) were housed in individually ventilated cages at room temperature of 22°C ± 2°C, humidity at 55 ± 5%, with a 12:12-h light: dark cycle and throughout the study period had access to water and standard chow ad libitum. The guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals, Government of India, were followed and the in-house animal ethics committee approved all experimental procedures.
Male db/db mice were divided into three groups of 10 animals each with similar body weight and plasma glucose (mean ± S.E.M.) levels. Mice were treated with 0.5% carboxy methyl cellulose (CMC), rosiglitazone (5 mg/kg, p.o.), or NFAT-133 (100 mg/kg, i.p.) twice daily for 10 days. Body weight was measured daily. On day 10, one hour after the last dose, mice were fasted for four hours so as to minimize the variability between the animals. Blood was collected from the fasted mice, plasma was separated by centrifugation (6000 g at 4°C for 7 minutes) and glucose was estimated using a biochemistry auto analyzer (Hitachi Science Systems Limited, Ibaraki, Japan). A small aliquot of separated plasma was stored at -20°C for estimation of insulin using an ELISA assay kit (Linco Research). Immediately after blood sample collection, mice were sacrificed, livers excised and weighed. The data was reproducible in two independent experiments.
All the results are expressed as Mean ± S.E.M. Statistical analysis was done using GraphPad Prism 4 (version 4.03, GraphPad Software, Inc., CA, USA). For comparison between means of more than two groups one-way analysis of variance (ANOVA) followed by Dunnett's post-hoc analysis was employed.
Taxonomy of the Streptomyces strain PM0324667
Effect of the ethyl acetate extract of PM0324667 on glucose uptake in differentiated skeletal muscle cells
Isolation and characterization of NFAT-133
Effect of the ethyl acetate extract (111/27/E.A) of Streptomyces strain PM034667 and fractions 1111/38/1, 2 and 3 on glucose uptake in differentiated L6 myotubes
Glucose uptake in L6 myotubes*
(Fold increase over control at 3 μg/ml)
Rosiglitazone (4 μg/ml)
NFAT-133 was isolated by fermentation of the Streptomyces culture PM0324667 using optimized fermentation media and conditions as described in the experimental section. The biological activity coincided with the profile on a LC-ESI positive ion mass spectrum which showed a distinct peak m/z at 299 [M + Na]+ suggesting a molecular weight of the compound was 276 (Additional file 1). The compound was a pale yellow viscous oil with a UV absorption maxima of 212 nm, and molecular formula; C17H24O3.
NMR assignment for NFAT-133 in CDCl3
The1H NMR (Additional file 5) and COSY spectra suggested the presence of trisubstituted benzene ring with coupled proton signal at δ 7.2 (C-5), 7.07 (C-7) and 7.05 (C-8). A proton signal at δ2.3 singlets was assigned as Me group at C-6 position. The olefinic protons at C-3, C-4 and the only CH2 unit at C-1 were well correlated in COSY spectrum.
Effect of NFAT-133 treatment in vitro
Effect of NFAT-133 on PPAR-γ activity in human PPAR-γ receptor transfected CV1 cells
% hPPAR-γ activation
NFAT-133, 100 μM*
NFAT-133, 30 μM*
Rosiglitazone, 1 μM*
Effect of NFAT-133 treatment in vivo
Pharmacokinetic (PK) profile of NFAT-133 (30 mg/kg) administered orally and intraperitoneally in female db/db mice
T max (h)
C max (μg/ml)
C max (μM)
Effect of NFAT-133 and rosiglitazone on the body weight and liver weight in response to treatment in db/db mice
% Change in body weight
% Change in liver weight
6.0 ± 0.6
2.3 ± 0.1
Rosiglitazone, 5 mg/kg, po
14.6 ± 0.8**
2.6 ± 0.1*
NFAT-133, 100 mg/kg, ip
-1.1 ± 1.2**
2.0 ± 0.1*
Streptomyces pactum was known to produce antitumor compounds like pactamycin (Bhuyan 1962), vasodilating secondary metabolite like actinopyrones A, B and C (Yano et al. 1986), NADH oxidase inhibitors such as piericidin class of compounds (Matsumoto et al. 1987), and polyetheric antibiotics such as lonomycin (Hamill et al. 1989). Similarly Streptomyces parvisporogenes has been reported to synthesize polyenic antifungal agent like Antibiotic PA 616 (Chas 1960) and pepstatin class of compounds which are pepsin inhibitors (Umezawa 1970). There has been no report of NFAT-133 or similar class of compounds isolated from strains of Streptomyces pactum or Streptomyces parvisporogenes to date.
Our screening program for new compounds has yielded the compound NFAT-133. NFAT-133 elicits a significant and dose dependent glucose uptake response in differentiated L6 cells. In skeletal muscles the glucose uptake is mediated by activation of the transporter molecule GLUT4 (Wijesekara et al. 2006). GLUT4 activation is controlled by regulatory proteins which activate either the AMPK or PI3K pathway. Several natural products have been earlier reported to elicit similar responses in L6 skeletal muscle cells. We have shown that the plant extracts Aegle marmelos and Syzygium cumini stimulate glucose uptake in skeletal muscle by augmenting the activity of the regulatory molecules; phosphatidyl inositol-3-kinase (PI3K), AMPK and GLUT-4 (Anandharajan et al. 2006). Ganoderma lucidum extract stimulates glucose uptake in L6 rat skeletal muscle cells by stimulating the activity of PI3K as well as a PI3K independent pathway which is controlled by the AMP activated protein kinase (Kamei et al. 2002; Jung et al. 2006; Cheng et al. 2006). It is possible that NFAT-133 may be mediating its effects through some of these pathways since the compound did not significantly activate PPAR-γ. PPAR-γ is a major signaling molecule that is implicated in glucose uptake by skeletal muscle cells in vivo. However, activation of this receptor has several drawbacks. This has been noted especially in the case of thiazolidinediones which are very successful in reducing the clinical symptoms of diabetes. However, their major drawback is that they mediate their effects through activation of the PPAR-γ family of nuclear receptors. The most significant side effects of this group of compounds include weight gain, hepatotoxicity and cardiovascular manifestations. The fact that our compound NFAT-133 did not induce glucose uptake through activation of PPAR-γ is significant. Hence, we evaluated the efficacy of the molecule in reducing systemic blood glucose levels in db/db mice. Our data showed that NFAT-133 reduces systemic blood glucose levels. This was correlated with a simultaneous decrease in plasma insulin. Though actinomycetes produce few but prominent antidiabetic compounds, our report of NFAT-133 activity has revealed that further exploration of actinomycetes group will provide an avenue for discoveries leading to new medicines for diabetes. In conclusion, we found that NFAT-133, a natural product isolated from Streptomycetes, stimulates glucose uptake in L6 myotubes. This mechanism might explain its antihyperglycemic effects in diabetic animals.
Antihyperglycemic effects of the Streptomycetes compound NFAT-133 has been revealed by in vitro and animal studies.
The authors are very grateful to Somesh Sharma (Managing Director, Piramal Life Sciences Limited), H. Sivaramkrishnan (President, Piramal Life Sciences Limited), and Arun Balakrishnan (Vice President, External Liaison and Screening, Piramal Life Sciences Limited) for the encouragement and approvals towards this work and the publication.
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