Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13568-023-01644-6/MediaObjects/13568_2023_1644_Fig3_HTML.png)
Immunomodulatory effects of polysaccharide on H22 malignant ascites mice model A Immunohistochemistry was performed of liver sample tissues to observed Foxp3 and Stat3 expression in mice model and observed a significant decrease in gene expression in a dose-dependent manner. Images were captured at the 40 × lens. B Cytokines level was checked by performing Elisa such as IL-2, TNFα, and INFγ was observed to have a significant increase in serum in a dose-dependent manner. C Western blot analysis was performed to observe protein expression of Foxp3 and Stat3 and results show a significant dose-dependent decrease in expression. *p < 0.05, **p < 0.01, ***p ≤ 0.001. *PPO (Polysaccharide from Pleurotus ostreatus), *CTX (Cyclophosphamide)